Detection of dengue virus serotype 3 in Cajamarca, Peru: Molecular diagnosis and clinical characteristics
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Authors
Aguilar-Luis, MiguelCarrillo-Ng, Hugo
Kym, Sungmin
Silva-Caso, Wilmer
Verne, Eduardo
Del Valle, Luis
Bazn-Mayra, Jorge
Zavaleta-Gavidia, Victor
Cornejo-Pacherres, Daniel
Tarazona-Castro, Yordi
Aquino-Ortega, Ronald
Cornejo-Tapia, Angela
Valle-Mendoza, Juana
Issue Date
2021-10-01
Metadata
Show full item recordPublisher
Wolters Kluwer Medknow PublicationsJournal
Asian Pacific Journal of Tropical MedicineDOI
10.4103/1995-7645.326257Additional Links
https://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=10;spage=456;epage=462;aulast=Aguilar-Luis;type=0Abstract
Objective: To describe and molecularly characterize an outbreak of dengue virus (DENV) infection in Cajamarca, an Andean region in Peru. Methods: A total of 359 serum samples from patients with acute febrile illness were assessed for the presence of DENV via RT-PCR, ELISA NS1, IgM and IgG in Cajamarca, Peru from January 2017 to June 2017. The evaluation of the different diagnostic tests and their applicability was performed. Results: Dengue virus was detected in 24.7% of samples by RTPCR. Meanwhile, serological analysis detected 30.3% positive cases via ELISA NS1 antigen, 16.7% via ELISA IgG and 9.7% via ELISA IgM. Most of the cases corresponded to DENV-3 (77.5%). The use of RT-PCR performed better in primary infections (P<0.01), while detection of ELISA IgM performed better in secondary infections (P<0.01). The combination of NS1 and IgM performed better than the other assays in detecting primary (92.5%) and secondary infections (96.6%). The most frequent symptoms associated with fever were headaches, myalgias, and arthralgias across all groups. Conclusions: We report an important outbreak of dengue infection caused by DENV-3 in Cajamarca, Peru. Our findings encourage the use of NS1 antigen and IgM co-detection. These findings demonstrate an increasing expansion of DENV-3 in Peru and highlight the importance of molecular diagnosis and serotype characterization among the clinically defined dengue cases to strengthen the Peruvian epidemiological surveillance.Type
info:eu-repo/semantics/articleRights
info:eu-repo/semantics/openAccessAttribution-NonCommercial-ShareAlike 4.0 International
Language
engISSN
19957645Sponsors
Ministry of Health and Welfareae974a485f413a2113503eed53cd6c53
10.4103/1995-7645.326257
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- Creative Commons