Evaluación in vitro de la actividad fungicida y fungistática del extracto metanólico de la minthostachys mollis (muña) sobre cepa de candida albicans ATCC®1023
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Issue Date
2018-11-20
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1. Neyra Espinoza LCJ, Armas Gálvez NM. Evaluación in vitro de la actividad fungicida y fungistática del extracto metanólico de la minthostachys mollis (muña) sobre cepa de candida albicans ATCC®1023 [Internet]. [Lima, Perú]: Universidad Peruana de Ciencias Aplicadas (UPC); 2018 [cited 2019 Feb 28]. doi: https://doi.org/10.19083/tesis/625175DOI
10.19083/tesis/625175Abstract
Objetivo: Evaluar in vitro la actividad fungistática y fungicida del extracto metanólico de Minthostachys Mollis (Muña) sobre cepas de Candida albicans. Materiales y Métodos: Se realizaron extractos metanólicos de las hojas, tallos y raíces de Minthostachys mollis. La actividad fungistática de los extractos frente a cepas de Candida albicans fue evaluado por medio de la técnica de difusión en agar. La concentración mínima inhibitoria (CMI) del extracto se determinó por el método de microdilución y la citotoxicidad usando la línea celular MDCK. Resultados: El extracto de hojas y tallo de Minthostachys mollis mostró mayor actividad fungistático frente a Candida albicans, observandose halos de inhibición de 47.72±6.67mm y 46.58±6.42mm respectivamente, no encontrando diferencia estadísticamente significativa entre ambos extractos. La CMI fue de 46.87mg/ml, 93.75mg/ml y 1500mg/ml para hojas, tallos y raíces respectivamente. Conclusiones: Se demostró que el extracto metanólico de Minthostachys mollis tienen actividad fungistática y fungicida contra cepas de Candida albicans. Ninguno de los extractos resultó ser tóxico sobre líneas celulares.Objective: to evaluate in vitro the fungistatic and fungicidal activity of the methanolic extract of Minthostachys Mollis (Muña) on strains of Candida albicans. Methods: Three in vitro methanolic extracts of the leaves, stems and roots of Minthostachys mollis were made. The fungistatic activity of the extracts against Candida albicans strains was evaluated by means of the agar diffusion technique. Minimun inhibitory concentration (MIC) was determined by the microdilution method and cytotoxicity using the MDCK cell line. Results: The extracts that obtained the greatest fungistatic activity at 24 hours were those of leaves and stems against Candida albicans, halos of 47.72 ± 6.67mm and 46.58 ± 6.42mm respectively were obtained, finding no statistically significant difference. The MIC for leaves was 46.87mg / ml, for stems was 93.75mg / ml and 1500mg / ml for roots. Conclusions: It was demonstrated that the methanolic extracts of Minthostachys mollis have fungistatic and fungicidal activity against strains of Candida albicans. None of the extracts turned out to be toxic on cell lines at low concentrations.
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info:eu-repo/semantics/bachelorThesisRights
info:eu-repo/semantics/openAccessAttribution-NonCommercial-ShareAlike 3.0 United States
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spaae974a485f413a2113503eed53cd6c53
10.19083/tesis/625175
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