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dc.contributor.advisorDel Valle Mendoza, Juana Mercedeses
dc.contributor.authorManrique Holguín, Paola Cecibel*
dc.date.accessioned2018-02-21T01:58:30Z
dc.date.available2018-02-21T01:58:30Z
dc.date.issued2017-12-14
dc.identifier.citationManrique Holguín, P. C. ( 0000-0001-8210-6922 ). (2017). Evaluación in vitro del efecto antimicrobiano y citotóxico del extracto metanólico dracontium loretense (jergón sacha) sobre cepas de candida albicans (ATCC®10231TM), Streptococcus mutans (ATCC®25175TM) y Streptococcus sanguinis (ATCC®10556TM). Universidad Peruana de Ciencias Aplicadas (UPC). Retrieved from http://hdl.handle.net/10757/622864es_PE
dc.identifier.urihttp://hdl.handle.net/10757/622864
dc.description.abstractObjective: The aim to this study was to evaluate the antimicrobial and cytotoxic effect of the methanol extract of Dracontium loretense (Jergon Sacha) against Candida albicans (ATCC®10231), Streptococcus mutans (ATCC®25175) and Streptococcus sanguinis (ATCC®25175). Materials and Methods: The methanolic extract of Dracontium loretense was prepared at different concentrations. The antimicrobial activity of Dracontium loretense was evaluated on strains of Candida albicans, Streptococcus mutans and Streptococcus sanguinis by the agar diffusion method. The cytotoxicity of the extract (CC50) was determined using the MDCK cell line. Results: The results showed that the methanolic extract of Dracontium loretense had inhibitory effect on Streptococcus mutans and Streptococcus sanguinis with inhibition halos of 14.10 ± 0.65mm and 15.58 ± 0.43mm, respectively. However, no inhibitory effect was observed on Candida albicans. The minimum inhibitory concentration (MIC) of the methanolic extract of Dracontium loretense on strains of Streptococcus mutans was 500 μg/ml. While the MIC on Streptococcus sanguinis was 62.5μg / ml. The cytotoxicity of the methanolic extract of Dracontium loretense on the MDCK cell line was evaluated in a range of 0 to 900 μg / ml, showing that it is not cytotoxic in high concentrations. Conclusion: The experimental findings showed that Dracontium loretense (Jergon Sacha) presented an antibacterial effect on the strains of Streptococcus mutans and Streptococcus sanguinis. However, it did not present an antifungal effect on strains of Candida albicans. The extract of this plant is not cytotoxic even in high concentrations.
dc.description.uriTesises_PE
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dc.publisherUniversidad Peruana de Ciencias Aplicadas (UPC)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.titleEvaluación in vitro del efecto antimicrobiano y citotóxico del extracto metanólico dracontium loretense (jergón sacha) sobre cepas de candida albicans (ATCC®10231™), Streptococcus mutans (ATCC®25175™) y Streptococcus sanguinis (ATCC®10556™)es
dc.typeinfo:eu-repo/semantics/bachelorThesises
thesis.degree.grantorUniversidad Peruana de Ciencias Aplicadas (UPC). Facultad de Ciencias de la Saludes_PE
thesis.degree.levelLicenciaturaes_PE
thesis.degree.disciplineOdontologíaes_PE
thesis.degree.nameCirujano dentistaes_PE
refterms.dateFOA2018-06-18T23:08:22Z
html.description.abstractObjetivo: Evaluar in vitro la actividad antimicrobiana y citotóxica del extracto metanólico de Dracontium loretense (Jergón Sacha) sobre cepas de Candida albicans (ATCC ® 10231™), Streptococcus mutans (ATCC®25175) y Streptococcus sanguinis (ATCC®25175). Materiales y Métodos: Se preparó el extracto metanólico de Dracontium loretense a diferentes concentraciones. Se evaluó la actividad antimicrobiana de Dracontium loretense sobre cepas de Candida albicans, Streptococcus mutans y Streptococcus sanguinis mediante el método difusión en agar. La citotoxicidad del extracto (CC50) se determinó haciendo uso de la línea celular MDCK. Resultados: El extracto metanólico de Dracontium loretense tuvo efecto inhibidor sobre Streptococcus mutans y Streptococcus sanguinis con halos de inhibición de 14.10 ± 0.65mm y 15.58 ± 0.43mm, respectivamente. Sin embargo, no se observó efecto inhibidor sobre Candida albicans. La concentración mínima inhibitoria (CMI) del extracto metanólico de Dracontium loretense sobre las cepas de Streptococcus mutans fue de 500µg/ml. Mientras que la CMI sobre Streptococcus sanguinis fue de 62.5µg/ml. La citotoxicidad de este extracto sobre la línea celular MDCK se evaluó en un rango de 0 a 900µg/ml, mostrando que no es citotóxico en altas concentraciones. Conclusiones: Se observó que Dracontium loretense (Jergón Sacha) presentó efecto antibacteriano sobre las cepas de Streptococcus mutans y Streptococcus sanguinis. Sin embargo, no presentó efecto antimicótico sobre cepas de Candida albicans. El extracto de esta planta no es citotóxico aún en altas concentraciones
html.description.abstractObjective: The aim to this study was to evaluate the antimicrobial and cytotoxic effect of the methanol extract of Dracontium loretense (Jergon Sacha) against Candida albicans (ATCC®10231), Streptococcus mutans (ATCC®25175) and Streptococcus sanguinis (ATCC®25175). Materials and Methods: The methanolic extract of Dracontium loretense was prepared at different concentrations. The antimicrobial activity of Dracontium loretense was evaluated on strains of Candida albicans, Streptococcus mutans and Streptococcus sanguinis by the agar diffusion method. The cytotoxicity of the extract (CC50) was determined using the MDCK cell line. Results: The results showed that the methanolic extract of Dracontium loretense had inhibitory effect on Streptococcus mutans and Streptococcus sanguinis with inhibition halos of 14.10 ± 0.65mm and 15.58 ± 0.43mm, respectively. However, no inhibitory effect was observed on Candida albicans. The minimum inhibitory concentration (MIC) of the methanolic extract of Dracontium loretense on strains of Streptococcus mutans was 500 μg/ml. While the MIC on Streptococcus sanguinis was 62.5μg / ml. The cytotoxicity of the methanolic extract of Dracontium loretense on the MDCK cell line was evaluated in a range of 0 to 900 μg / ml, showing that it is not cytotoxic in high concentrations. Conclusion: The experimental findings showed that Dracontium loretense (Jergon Sacha) presented an antibacterial effect on the strains of Streptococcus mutans and Streptococcus sanguinis. However, it did not present an antifungal effect on strains of Candida albicans. The extract of this plant is not cytotoxic even in high concentrations.


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