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dc.contributor.authorPalma, Noemíes
dc.contributor.authorPons, Maria J.es
dc.contributor.authorGomes, Cláudiaes
dc.contributor.authorMateu, Judites
dc.contributor.authorRiveros, Maribeles
dc.contributor.authorGarcía, Wilfredoes
dc.contributor.authorJacobs, Janes
dc.contributor.authorGarcía, Coralithes
dc.contributor.authorOchoa, Theresa J.es
dc.contributor.authorRuiz, Joaquimes
dc.date.accessioned2017-10-20T20:07:53Z
dc.date.available2017-10-20T20:07:53Z
dc.date.issued2017-12
dc.identifier.citationResistance to quinolones, cephalosporins and macrolides in Escherichia coli causing bacteraemia in Peruvian children 2017, 11:28 Journal of Global Antimicrobial Resistancees
dc.identifier.issn22137165
dc.identifier.doi10.1016/j.jgar.2017.06.011
dc.identifier.urihttp://hdl.handle.net/10757/622267
dc.descriptionEl texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.es_PE
dc.description.abstractObjectives To characterise the β-lactam, quinolone and macrolide resistance levels and mechanisms in 62 Escherichia coli isolates causing bacteraemia in Peruvian children. Methods Minimum inhibitory concentrations (MICs) of ciprofloxacin, nalidixic acid (NAL) and azithromycin were determined in the presence and absence of Phe-Arg-β-naphthylamide. Susceptibility to other 14 antimicrobial agents was also established. Extended-spectrum β-lactamases (ESBLs) were identified, and mutations in gyrA and parC as well as the presence of transferable mechanisms of quinolone resistance (TMQR) and macrolide resistance (TMMR) were determined. Results Fifty isolates (80.6%) were multidrug-resistant. High proportions of resistance to ampicillin (93.5%), NAL (66.1%) and trimethoprim/sulfamethoxazole (66.1%) were observed. No isolate showed resistance to carbapenems and only two isolates were resistant to nitrofurantoin. Twenty-seven isolates carried ESBL-encoding genes: 2 blaSHV-12; 13 blaCTX-M-15; 4 blaCTX-M-2; 6 blaCTX-M-65; and 2 non-identified ESBLs. Additionally, 27 blaTEM-1 and 9 blaOXA-1-like genes were detected. All quinolone-resistant isolates showed target mutations, whilst TMQR were present in four isolates. Efflux pumps played a role in constitutive NAL resistance. The association between quinolone resistance and ESBL production was significant (P = 0.0011). The mph(A) gene was the most frequent TMMR (16 isolates); msr(A) and erm(B) genes were also detected. Only one TMMR-carrying isolate [presenting mph(A) and erm(B) concomitantly] remained resistant to azithromycin when efflux pumps were inhibited. Conclusions A variety of ESBL-encoding genes and widespread of blaCTX-M-15 in Lima has been shown. The role of efflux pumps in azithromycin resistance needs to be further evaluated, as well as effective control of the use of antimicrobial agents. © 2017 International Society for Chemotherapy of Infection and Cancer
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherElsevier Ltdes
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2213716517301339es
dc.rightsinfo:eu-repo/semantics/restrictedAccesses
dc.subjectAntimicrobial resistancees
dc.subjectBacteraemiaes
dc.subjectExtended-spectrum β-lactamase (ESBLes
dc.subjectMacrolide resistancees
dc.subjectPerues
dc.subjectQuinolone resistancees
dc.titleResistance to quinolones, cephalosporins and macrolides in Escherichia coli causing bacteraemia in Peruvian childrenes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.journalJournal of Global Antimicrobial Resistancees
dc.description.fundingJR has a fellowship from the program I3SNS of the ISCIII [grant no. CES11/012]; MJP has a postdoctoral fellowship from CONCYTEC/FONDECYT [grant no. CG05-2013-FONDECYT]; CG had a predoctoral grant from the ISCIII [FI12/00561]. This work was supported by: Agencia Española de Cooperación Internacional para el Desarrollo (AECID), Spain, Programa de Cooperación Interuniversitaria e Investigación Científica con Iberoamérica [D/019499/08, D/024648/09, D/030509/10 and A1/035720/11]; Spanish Network for the Research in Infectious Diseases [REIPI RD12/0015]; and Generalitat de Catalunya, Departament d'Universitats, Recerca i Societat de la Informació [2014 SGR 26].es_PE
dc.description.peerreviewRevisión por pareses_PE
dc.contributor.emailjoruiz.trabajo@gmail.comes_PE
refterms.dateFOA2018-06-15T09:12:22Z
html.description.abstractObjectives To characterise the β-lactam, quinolone and macrolide resistance levels and mechanisms in 62 Escherichia coli isolates causing bacteraemia in Peruvian children. Methods Minimum inhibitory concentrations (MICs) of ciprofloxacin, nalidixic acid (NAL) and azithromycin were determined in the presence and absence of Phe-Arg-β-naphthylamide. Susceptibility to other 14 antimicrobial agents was also established. Extended-spectrum β-lactamases (ESBLs) were identified, and mutations in gyrA and parC as well as the presence of transferable mechanisms of quinolone resistance (TMQR) and macrolide resistance (TMMR) were determined. Results Fifty isolates (80.6%) were multidrug-resistant. High proportions of resistance to ampicillin (93.5%), NAL (66.1%) and trimethoprim/sulfamethoxazole (66.1%) were observed. No isolate showed resistance to carbapenems and only two isolates were resistant to nitrofurantoin. Twenty-seven isolates carried ESBL-encoding genes: 2 blaSHV-12; 13 blaCTX-M-15; 4 blaCTX-M-2; 6 blaCTX-M-65; and 2 non-identified ESBLs. Additionally, 27 blaTEM-1 and 9 blaOXA-1-like genes were detected. All quinolone-resistant isolates showed target mutations, whilst TMQR were present in four isolates. Efflux pumps played a role in constitutive NAL resistance. The association between quinolone resistance and ESBL production was significant (P = 0.0011). The mph(A) gene was the most frequent TMMR (16 isolates); msr(A) and erm(B) genes were also detected. Only one TMMR-carrying isolate [presenting mph(A) and erm(B) concomitantly] remained resistant to azithromycin when efflux pumps were inhibited. Conclusions A variety of ESBL-encoding genes and widespread of blaCTX-M-15 in Lima has been shown. The role of efflux pumps in azithromycin resistance needs to be further evaluated, as well as effective control of the use of antimicrobial agents. © 2017 International Society for Chemotherapy of Infection and Cancer


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