• Evaluación in vitro del efecto antibacteriano y citotóxico del extracto metanólico de semilla y pulpa de la Myrciaria dubia (camu camu) sobre cepas de Streptococcus mutans (ATCC 25175) y Streptococcus sanguinis (ATCC 10556)

      Mayta Tovalino, Frank Roger; Del Valle Mendoza, Juana Mercedes; Camere Colarossi, Rosella Vanina (Universidad Peruana de Ciencias Aplicadas (UPC)PE, 04/11/2015)
      Objective: The aim of this study was to evaluate the antibacterial and cytotoxic effect of the methanol extract of the seed and pulp of Myrciaria dubia (camu camu) against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556). Materials and Methods: For the present in vitro experimental study two methanolic extracts were prepared from the seed and pulp of Myrciaria dubia (camu camu) to be tested against Streptococcus mutans and Streptococcus sanguinis. Ten independents tests were prepared for each type of extract, using Clorhexidine at 0.12% solution as a positive control. The method used was agar diffusion test preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 hours at 37°C, after this time the growth inhibition halo was analysed in millimeters using a vernier. Meanwhile, the Minimum Inhibitory Concentration (MIC) and the cytotoxic effect (CC50) over Jurkat T cell line was found. Results: The methanolic seed extract had more antibacterial effect over Streptococcus mutans with an inhibitory halo of 21.36mm ± 6.35, while the pulp had 16.20mm ± 2.08. For the Streptococcus sanguinis group the inhibitory halo were 19.21mm ± 5.18 and 19.34mm ± 2.90 for the methanolic seed and pulp extract respectively. The MIC for the pulp extract was 125µg/ml for both strains, whereas for the seed antibacterial activity was observed even at low concentrations. Finally the CC50 for the seed extract was at a higher concentration than 800 µg/ml and 524.37µg/ml for the pulp exactract. Conclusions: The methanolic seed and pulp extract of Myrciaria dubia (camu camu) had antibacterial effect against Streptococcus mutans and Streptococcus sanguinis. These extracts were not cytotoxic.
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