Browsing Odontologia by Author "Arévalo Híjar, Olga Lucía"
Efecto antibacteriano y citotóxico de dos extractos metanólicos a base de Moringa oleifera (moringa) y Azadirachta indica (neem) sobre cepas de Enterococcus faecalisGonzáles Soto, Néstor; Del Valle Mendoza, Juana; Arévalo Híjar, Olga Lucía (Universidad Peruana de Ciencias Aplicadas (UPC), 22/02/2017)Objective: To evaluate in vitro the antibacterial and cytotoxic effect of two methanolic extracts of Azadirachta indica and Moringa oleifera on strains of Enterococcus faecalis (ATCC 29212). Methods: The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against strains of Enterococcus faecalis were evaluated by agar diffusion technique (gel perforation). Minimum inhibitory concentration (MIC) was determined by the microdilution method and cytotoxicity using the MDCK cell line. Results: The methanolic extract that obtained the highest antibacterial effect in 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, obtaining a halo of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 μg / ml. The bactericidal effect for Azadirachta indica extract was 25 μg / ml and for Moringa oleifera extract it was 75 μg / ml. Conclusions: It was demonstrated that the methanolic extracts of Azadirachta indica and Moringa oleifera have an antibacterial effect against strains of Enterococcus faecalis at 24 and 48 hours. Neither extract was toxic to cell lines at low concentrations.Acceso restringido temporalmente