Browsing Tesis by Issue Date "01/03/2017"
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Índice de masa corporal asociado al daño en pacientes con Lupus Eritematoso Sistémico del Hospital Nivel IV Guillermo Almenara Irigoyen durante los años 2012- 2015(Universidad Peruana de Ciencias Aplicadas (UPC), 01/03/2017)Introduction: To determine the association between body mass index (BMI) and damage in patients with systemic lupus erythematosus (SLE). Methods: This is a Cross-sectional analytical study. The main variables were body mass index (BMI) <25 kg/m2 (normal) and ≥25 kg/m2 (overweight and obesity) and damage measured by SLICC-ACR (Systemic Lupus International Collaborating Clinics/American College of Rheumatology) damage index. Other variables were sociodemographic and laboratory features, drug use and subtotal percentage of fat, leg fat, trunk fat and trunk/leg fat ratio. Results: There were studied, 309 patients. Women were 287 (92, 88%) and the average age was 42 ± 12, 98 years. The number of patients with BMI ≥ 25 kg/m2 was 194 (62, 78%). The prevalence of damage was 149 (48, 22%) patients. In the univariate analysis, the variables associated were advanced age, increased year of education, increased disease time, hypertriglyceridemia, hypercholesterolemia, low levels of HDL (<40mg/dl), higher creatinine levels, increased prednisone use, diagnosis of hypertension, actual use of immunosuppressive drugs and higher trunk/leg fat ratio. In the multivariate analysis, the associated variables were advanced age (PR:1,02; CI95%1,01-1,03), actual use of immunosuppressive drugs (PR:1,49; CI95% 1,02-2,19) and greater proportion of fat ratio trunk/leg (PR:1,24; CI95%:1,11-1,39). There was no association between BMI and damage. Conclusions: In the present study, no association between BMI (overweight/obesity) and damage was found. However, we obtained the association with the use of immunosuppressive drugs, higher trunk/leg fat ratio and older age.Acceso restringido temporalmente
Microfiltración coronal de un material de restauración temporal experimental para su uso en endodoncia(Universidad Peruana de Ciencias Aplicadas (UPC), 01/03/2017)Coronal microfiltration caused by temporary restorative materials is considered one of the causes of failure in endodontic treatments. As a result, in recent years it has been sought to create a temporary restoration material that is capable of avoiding this problem. Objective: compare in vitro coronal microfiltration of an "experimental cement" and four temporary restorative materials used in endodontics. Materials and methods: Class I cavities were made in 90 premolars, divided into 10 groups (n = 9) and evaluated in two time periods (1 and 2 weeks. The experimental cement was elaborated, later the coronal sealing of the dental pieces was carried out, with experimental Cement,F (VOCO), Eugenato (MOYCO), Ketac ™ Molar Easymix 3M (ESPE) and Coltosol®F were used for the preparation of the experimental. The specimens were immersed in Chinese ink (Pelikan) for 1 and 2 weeks. Microfiltration at the dentinal wall-temporal restoration interface was evaluated using a stereomicroscope (Leica Microsystems LAS EZ version 2.0.0). Results: We found statistically significant differences (p <0.05) when coronal microfiltration of the five temporary restoration materials were compared, according to the ink exposure time (1 and 2 weeks). In both weeks, the experimental cement presented less filtration than Coltosol® F and Ketac ™ Molar Easymix 3M (ESPE). On the other hand, when comparing the coronal microfiltration of the experimental cement with each of the temporary restoration materials, we found statistically significant differences for the experimental group - Ketac ™ Molar Easymix 3M (ESPE) in the first week (p <0.001) and in the second week (p = 0.003). Conclusion: Experimental Cement presented lower microfiltration than Coltosol® F and Ketac ™ Molar EasyMix 3M (ESPE). However, none of the materials were able to prevent microfiltration totally.Acceso restringido temporalmente
New sensor protein for phosphate dissociation during bacterial mRNA translation(Universidad Peruana de Ciencias Aplicadas (UPC), 01/03/2017)The translation initiation process is an important checkpoint that assures the correct protein production. Within this phase, Initiation Factor IF2 plays an important role along all early and late steps of the process. During late reactions, IF2 enhances the joining of the 50S subunit to the 30S Initiation Complex (IC) and positions initiator tRNA in the 70S IC. Concomitantly, IF2 hydrolyses a GTP molecule which led to propose that the active hydrolysis of GTP stimulates both above events. However, recent mutagenic studies of IF2 showed that inhibiting its GTP hydrolytic activity does not compromise the overall translation initiation process. Moreover, biochemical studies indicate that the dissociation of inorganic phosphate (Pi) is a late event, prior to the release of IF2. These findings indicated that it is the dissociation of Pi that weakens the interaction of IF2 with the ribosome. However, the GTP hydrolysis reaction is energetically favorable and may actively drive factor release. To elucidate which of the above postulates describe more accurately IF2 dependent reactions, here we design, produce and test a novel recombinant fluorescent phosphate binding sensor that specifically binds nearby the exit point of Pi during protein translation. This protein chimeras could evidence whether the IF2 dissociation is catalyzed by the Pi dissociation after GTP hydrolysis or by the reaction per se. Furthermore, the system provides a novel platform to study and systematically screen for new antimicrobial compounds.Acceso restringido temporalmente