Recent Submissions

  • Coronal microleakage according to the temporary restorative materials used in endodontic treatment

    Prado, Luisa Stephanie Nohelya Junes; Garcia, Stefany Caballero; Salazar, Carolina Barragán; Soto, Néstor Gonzales (Editorial Ciencias Medicas, 2020-04-01)
    Introduction: Coronal microleakage caused by temporary restorative materials is viewed as one of the reasons for endodontic failure. Efforts have been made in recent years to create a temporary restorative material capable of preventing that problem. Objective: Compare in vitro coronal microleakage of an "experimental cement" and four temporary restorative materials used in endodontics. Methods: Class I cavities were made in 90 premolar teeth divided into 10 groups (n= 9) and evaluated at two time periods (1 and 2 weeks). The "experimental cement" was prepared and then coronal sealing of the teeth was performed with the experimental Cement, F (VOCO), Eugenato (MOYCO), Ketac™ Molar Easymix 3M (ESPE) and Coltosol® F. The pieces were submerged in India ink (Pelikan)for 1 and 2 weeks. Microleakage was evaluated on the interface dentin wall / temporary restoration using a stereo microscope (Leica Microsystems LAS EZ version 2.0.0). Measurement was made in millimeters using the software LAS EZ version 2.0.0. Results: Statistically significant differences were found (p< 0.05) when comparing coronal microleakage in the five temporary restorative materials according to exposure time in ink (1 and 2 weeks). Conclusions: The experimental cement had less microleakage than Coltosol® F and Ketac™ Molar EasyMix 3M (ESPE). However, none of the four materials was capable of completely preventing microleakage.
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  • Screening and Assessment of Antimicrobial Susceptibility of Periodontopathic Bacteria in Peruvian Patients with Periodontitis: A Pilot Study

    Aguilar-Luis, Miguel Angel; Casas Apayco, Leslie; Tinco Valdez, Carmen; De Lama-Odría, María del Carmen; Weilg, Claudia; Mazulis, Fernando; Silva-Caso, Wilmer Gianfranco; Del Valle-Mendoza, Juana Mercedes (Hindawi Limited, 2021-01-01)
    Background. Severe periodontal disease is highly prevalent worldwide, affecting 20% of the population between the ages of 35 and 44 years. The etiological epidemiology in Peru is scarce, even though some studies describe a prevalence of 48.5% of periodontal disease in the general population. Periodontitis is one of the most prevalent oral diseases associated with site-specific changes in the oral microbiota and it has been associated with a socioeconomic state. This study aimed to determine the etiology and resistance profile of bacteria identified in a group of Peruvian patients with periodontal disease. Methods. Six subgingival plaque samples were collected from eight patients with severe periodontitis. Bacterial identification was carried out by an initial culture, PCR amplification, and subsequently DNA sequencing. We evaluated the antibiotic susceptibility by the disk diffusion method. Results. Variable diversity in oral microbiota was identified in each one of the eight patients. The bacterial genus most frequently found was Streptococcus spp. (15/48, 31.3%) followed by Rothia spp. (11/48, 22.9%), Actinomyces spp. (9/48, 18.8%), and Eikenella spp. (4/48, 8.3%). The most common species found was Rothia dentocariosa (8/48, 16.7%). The antimicrobial susceptibility assay varied according to the species tested; however, among all the isolates evaluated, Actinomyces naeslundii was resistant to penicillin and tetracycline; Eikenella corrodens was resistant to dicloxacillin; and Rothia dentocariosa was resistant to amoxicillin + clavulanic acid and metronidazole but also susceptible to trimethoprim-sulfamethoxazole. Conclusions. The most prevalent periodontal bacterium found in this study was Rothia dentocariosa. Specific antimicrobial therapy is required to improve the treatment outcomes of patients with periodontal disease and avoid antibiotic resistance.
  • Dimension and morphology of the mandibular condyle in Class I patients in cone beam computed tomography

    Bustamante, Carmen; Labrín, Vanessa; Casas-Apayco, Leslie; Ghersi-Miranda, Hugo (Universidad de Concepcion, 2020-01-01)
    To evaluate the anterior-posterior (A-P)/medial-lateral (M-L) dimension, and morphology of the mandibular condyle in patients aged 18 to 65 years with Class I skeletal pattern on Cone Beam Computed Tomography scans (CBCTs). Materials and Methods: Seventy one CBCTs were evaluated using RealScan 2.0 software. The dimension was determined by points A (most anterior in the sagittal plane), P (most posterior in the sagittal plane), M (most interior in the coronal plane), L (most exterior in the coronal plane). The morphology of the condyle was evaluated in two coronal and sagittal planes, being classified as: round, flat, convex or mixed. The size of the condyle was analyzed by descriptive statistics and the morphology by frequency distribution. For the bivariate analysis, the Student’s t-test was applied. Results: Measurements were obtained for the A-P diameter of the right condyle (RC) (8.72mm ± 1.25mm) and the left condylar (LC) (8.50mm ± 1.50mm), the M-L diameter of the RC (19.24mm ± 2.03mm) and the LC (18.97mm ± 1.87mm). There were significant differences in the male M-L dimension of the LC compared to the female (p=0.002). The most prevalent morphology of RC (35.21) and IQ (23.94) in the coronal plane was round.. Conclusion: The A-P dimension of the right and left condyle is similar in both genders; however, there are differences in the M-L dimension of the left male condyle. The most prevalent morphology of the right and left condyle was round in the sagittal plane with the exception of the coronal plane.
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  • In vitro comparison of the immediate adhesive strength in dentin of three universal adhesive systems

    Acurio-Benavente, Mariana; Kinoshita-Rivas, Haru; Acurio-Benavente, Paloma; Casas-Apayco, Leslie; (Universidad de Concepción, 2018-05)
    Objective: To compare the adhesive strength in dentin of three universal adhesive systems in vitro by means of the shear test. Materials and methods: Seventy-five bovine teeth were selected and cut. Dentin was exposed from the buccal surface of the crowns with 220 grit sandpaper, and samples were then inserted in transparent acrylic bases (15x10mm). The samples were randomly divided in 3 groups (n=25): G1-Universal adhesive system Scotchbond™ Universal (3M ESPE-USA); G2-Universal adhesive system Peak Universal Bond (Ultradent); G3-Universal adhesive system Tetric N-Bond (Ivoclar Vivadent). The adhesive procedures were carried out according to the instructions of each manufacturer and the restorative procedures were carried out with micro-cylinders (made of Tygon type tubing 0.79x1.5mm) of the composite resin Filtek™ Z350XT-A2 (3M ESPE-USA). The samples were incubated at 37ºC (+/-5ºC) for 24 hours. Adhesive strength was evaluated in a universal test machine by means of the shear test (0.5mm/ min, 500N) and the resulting fracture type was evaluated with a Dinolite digital microscope (x200). The results were analyzed by descriptive statistics (Mean±SD), and inferential statistics by a one-way ANOVA. Results: No statistically significant differences were found between the universal adhesive systems evaluated G1 (14.91±4.76), G2 (16.90±4.11) and G3 (17.34±4.04)/(p=0.114). Conclusions: The shear test resulted in similar values of immediate adhesive strength of the three universal adhesive systems used.
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  • Comentario sobre «Disfunción de la articulación temporomandibular en pacientes con artritis reumatoide»

    Bustamante-Flores, Carmen R.; Labrin-Valdiviezo, Vanessa; (Elsevier B.V., 2018-02)
    Cartas al editor
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  • Comment on “postoperative pain and flare-ups: Comparison of incidence between single and multiple visit pulpectomy in primary molars”

    Manrique, P.C; Castillo-Cabezudo, E.M. (Journal of Clinical and Diagnostic Research, 2018-01-01)
    Carta al editor
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  • Mandibular condyle dimensions in Peruvian patients with Class II and Class III skeletal patterns.

    Zegarra-Baquerizo, Hugo; Moreno-Sékula, Katica; Casas-Apayco, Leslie; Ghersi-Miranda, Hugo; (Universidad de Concepcion, 2017-10-26)
    Objective: To compare condylar dimensions of young adults with Class II and Class III skeletal patterns using cone-beam computed tomography (CBCT). Materials and methods: 124 CBCTs from 18-30 year-old patients, divided into 2 groups according to skeletal patterns (Class II and Class III) were evaluated. Skeletal patterns were classified by measuring the ANB angle of each patient. The anteroposterior diameter (A and P) of the right and left mandibular condyle was assessed from a sagittal view by a line drawn from point A (anterior) to P (posterior). The coronal plane allowed the evaluation of the medio-lateral diameter by drawing a line from point M (medium) to L (lateral); all distances were measured in mm. Results: In Class II the A-P diameter was 9.06±1.33 and 8.86±1.56 for the right and left condyles respectively, in Class III these values were 8.71±1.2 and 8.84±1.42. In Class II the M-L diameter was 17.94±2.68 and 17.67±2.44 for the right and left condyles respectively, in Class III these values were 19.16±2.75 and 19.16±2.54. Conclusion: Class III M-L dimensions showed higher values than Class II, whereas these differences were minimal in A-P.
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  • Overall assessment of responsiveness to change is just the very first step: a technical commentary on Abanto et al.'s study

    Aguilar-Huaman, Daniela M.; Caballero-García, Stefany; Pereyra-Elías, Reneé; Segura, Eddy R.; Abanto, Jenny; School of Dentistry; Universidad Peruana de CienciasAplicadas; Lima Perú; School of Dentistry; Universidad Peruana de CienciasAplicadas; Lima Perú; School of Dentistry; Universidad Peruana de CienciasAplicadas; Lima Perú; School of Dentistry; Universidad Peruana de CienciasAplicadas; Lima Perú; Universidade de Sao Paulo; San Paulo Brazil (Blackwell Publishing Ltd, 2017-05)
    Carta al Editor
  • Comment on “Referral of young children to dental personnel by primary care nurses”

    Maccha, L; Montenegro, H; Caballero-García, S; Pereyra Elías, Reneé; School of Dentistry; Universidad Peruana de Ciencias Aplicadas; Lima Perú; (John Wiley and Sons, 2017-02)
    Cartas al editor
    Acceso abierto
  • Comparación de la microfiltración del Ketac™ Molar e Ionofil Molar® con centrix y espátula TRA

    Basurto Sampedrano, Katherine Elizabeth; Barragán Salazar, Nathaly Carolina (2016)
    Introducción: El tratamiento restaurador atraumático, es utilizado como parte de los programas preventivos de salud pública, por ello es importante evaluar las técnicas de aplicación del material restaurador utilizando cemento ionómero de vidrio de autocurado. Objetivo: Comparar la microfiltración de los cementos ionoméricos Ketac™ Molar Easy Mix e Ionofil Molar® con dos técnicas de aplicación: jeringa centrix y espátula TRA. Materiales y métodos: Estudio experimental in vitro. Se utilizaron 52 dientes de bovino con preparaciones cavitarias. Las muestras se dividieron en 2 grupos según los cementos ionoméricos y según la técnica de aplicación. Se colocó el material restaurador siguiendo las indicaciones del fabricante. Todas las muestras fueron termocicladas, sumergidas en azul de metileno al 0,05% y seccionadas mediante un corte longitudinal. Se evaluó la microfiltración a través del software del esteromicroscopio. Para comparar las técnicas de aplicación según los cementos ionoméricos se utilizaron las pruebas estadísticas de t de Student y la prueba de U de Mann- Whitney. Resultados: La microfiltración de los cementos ionoméricos Ketac TM Molar Easy Mix e Ionofil Molar® según las técnicas de aplicación jeringa centrix y espátula TRA, no mostraron diferencias estadísticamente significativas (valor p>0,05). El cemento Ketac TM Molar Easy Mix y la técnica con la jeringa centrix y su respectiva combinación fueron los que presentaron menor microfiltración. Conclusión: Todos los dientes presentaron microfiltración y entre ellos el grupo KetacTM Molar Easy Mix® con la jeringa centrix mostraron menores valores de microfiltración
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  • Effect of simulated intraoral erosion and/or abrasion effects on etch-and-rinse bonding to enamel.

    Wang, Linda; Casas-Apayco, Leslie; Hipólito, Ana Carolina; Dreibi, Vanessa Manzini; Giacomini, Marina Ciccone; Bim Júnior, Odair; Rios, Daniela; Magalhães, Ana Carolina (American Journal of Dentistry, 2014-02)
    PURPOSE: To assess the influence of simulated oral erosive/abrasive challenges on the bond strength of an etch-and-rinse two-step bonding system to enamel using an in situ/ex vivo protocol. METHODS: Bovine enamel blocks were prepared and randomly assigned to four groups: CONT - control (no challenge), ABR - 3x/day-1 minute toothbrushing; ERO - 3x/day - 5 minutes extraoral immersion into regular Coca Cola; and ERO+ABR - erosive protocol followed by a 1-minute toothbrushing. Eight blocks were placed into an acrylic palatal appliance for each volunteer (n = 13), who wore the appliance for 5 days. Two blocks were subjected to each of the four challenges. Subsequently, all the blocks were washed with tap water and Adper Single Bond 2/Filtek Z350 were placed. After 24 hours, 1 mm2 beams were obtained from each block to be tested with the microtensile bond strength test (50 N load at 0.5 mm/minute). The data were statistically analyzed by one-way RM-ANOVA and Tukey's tests (alpha = 0.05). RESULTS: No difference was detected among the ABR, ERO, and CONT groups (P > 0.05). ERO+ABR group yielded lower bond strengths than either the ABR and ERO groups (P < 0.0113).
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  • Chlorhexidine does not improve but preserves bond strength to eroded dentin.

    Francisconi dos Rios, Luciana Fávaro; Calabria, Marcela Pagani; Casas-Apayco, Leslie; Honório, Heitor Marques; Carrilho, Marcela Rocha De Oliveira; Pereira, José Carlos; Wang, Linda (American Journal of Dentistry, 2015-02)
    PURPOSE: To evaluate the effect of aqueous solutions of chlorhexidine digluconate (CHX) in different concentrations on bond strength to eroded dentin up to 6 months, using normal dentin as a control. METHODS: Exposed flat dentin of extracted third molars was only ground with 600-grit SiC paper/1 minute (normal dentin - N), or subsequently eroded by a regular-cola soft-drink (eroded dentin - E). N and E were acid-etched, washed, dried and rehydrated with 1.5 μL, respectively, of distillated water (control - NC / EC); of 0.004% CHX (N0.004% / E0.004%); or of 2% CHX (N2% / E2%). Adper Single Bond 2 was applied in all specimens and resin composite buildups were constructed with Filtek Z350. Specimens were sectioned in beams, which were tested (μTBS) immediately or after 6 months of aging. RESULTS: Microtensile bond strength to eroded dentin was always significantly lower than that to normal dentin. Application of tested CHX solutions did not exert a significant effect immediately; however, after aging, the 2% CHX prevented abrupt bond strength loss both to eroded and normal dentin.
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  • A 12-month clinical trial examining the effects of a surface sealant on Class I composite resin restorations.

    Nahsan, Flavia Pardo Salata; Wang, Linda; Modena, Karin Silva; Francisconi Dos Rios, Luciana Fàvaro; Silva, Luciana Mendonça da; Calabria, Marcela Pagani; Casas-Apayco, Leslie; Mondelli, Rafael Francisco Lia (Chicago, Academy of General Dentistry, 2016-03)
    A split-mouth, double-blind trial evaluated the effects of a surface sealant on the clinical performance of Class I composite resin restorations. In 16 patients, 27 pairs of maxillary and mandibular molars or premolars with Class I carious lesions or unsatisfactory restorations were restored with composite resin. For each pair, 1 surface was sealed with surface sealant. Clinical evaluations of marginal integrity, marginal discoloration, anatomical form, and secondary caries were performed by 2 experienced operators using modified US Public Health Service criteria 1-2 weeks and 6 and 12 months after treatment. Data were analyzed with the McNemar test (P < 0.05). After 6 months, only 1 (4%) sealed restoration presented a Bravo rating for marginal integrity. After 12 months, the Bravo ratings for marginal integrity were 2 (7%) for sealed restorations and 1 (4%) for nonsealed restorations. Restorations received a score of Alfa for all other parameters at all time periods. There were no statistically significant differences within or between the sealed and nonsealed groups (P = 1.0). The use of a surface sealant did not improve the clinical performance of posterior composite resin Class I restorations.
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  • Role of chlorhexidine in bond strength to artificially eroded dentin over time.

    Francisconi dos Rios, Luciana Fávaro; Casas-Apayco, Leslie; Calabria, Marcela Pagani; Francisconi, Paulo Afonso Silveria; Borges, Ana Flávia Sanches; Wang, Linda (Quintessence Publishing Group, 2015-04)
    PURPOSE: To assess the long-term effect of a 2% aqueous chlorhexidine (CHX) solution on bond strength to artificially eroded dentin compared to sound dentin. MATERIALS AND METHODS: Flat mid-coronal dentin surfaces of extracted third molars (n = 28) were subjected only to grinding with a 600-grit SiC paper for 1 min (sound dentin S, n = 14) or additionally to erosive pH cycling with a cola-based soft-drink (eroded dentin E, n = 14). After acid etching, rinsing, and air drying, S and E were rehydrated with 1.5 μl of 2% CHX (S2%, n = 7; E2%, n = 7) or of distilled water (control SC, n = 7; EC, n = 7). Composite buildups were incrementally constructed with Filtek Z350 following Adper Single Bond 2 application. Specimens were sectioned into beams, which were subjected to microtensile testing immediately or after 6 or 12 months of aging. Fractured surfaces were observed under a digital microscope (50X magnification). Microtensile bond strength (μTBS) (MPa) was analyzed by three-way ANOVA and Tukey's tests (α = 0.05) and failure mode by the Kruskal-Wallis test (α = 0.05). RESULTS: Compared to sound dentin, eroded dentin was consistently related to lower μTBS. Immediately and after 12-month aging, the effect of CHX was insignificant, but it was significant after 6-month aging, when it conserved the bond strength to both eroded and sound dentin. The percentage of adhesive and mixed failures were equivalent, and significantly more frequent than cohesive failures, whether in dentin or in composite. CONCLUSION: The 2% CHX effect on bond strength conservation to both eroded and sound dentin was not found to be persistent.
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  • Feedback en educación clínica

    Ceccarelli Calle, Juan Francisco (Universidad Peruana Cayetano Heredia (UPCH), 2015-02-25)
    El feedback, también llamado retroalimentación, constituye un componente central en la educación médica. Desafortunadamente, los educadores médicos frecuentemente creen que brindan un adecuado feedback a sus alumnos. Sin embargo, diversos estudios muestran resultados contradictorios. El objetivo del presente documento es revisar los principales conceptos asociados al feedback en la educación clínica como parte del proceso de enseñanza–aprendizaje.
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  • Erosive cola-based drinks affect the bonding to enamel surface: an in vitro study

    Casas-Apayco, Leslie; Dreibi, Vanessa Manzini; Hipólito, Ana Carolina; Graeff, Márcia Sirlene Zardin; Rios, Daniela; Magalhaes, Ana Carolina; Buzalaf, Marília Afonso Rabelo; Wang, Linda (Faculdade De Odontologia De Bauru - USP, 2014-08-04)
    Objective: This study aimed to assess the impact of in vitro erosion provoked by different cola-based drinks (Coke types), associated or not with toothbrushing, to bonding to enamel. Material and methods: Forty-six bovine enamel specimens were prepared and randomly assigned into seven groups (N=8): C- Control (neither eroded nor abraded), ERO-RC: 3x/1-minute immersion in Regular Coke (RC), ERO-LC: 3x/1-minute immersion in Light Coke (LC), ERO-ZC: 3x/1-minute immersion in Zero Coke (ZC) and three other eroded groups, subsequently abraded for 1-minute toothbrushing (EROAB-RC, EROAB-LC and EROAB-ZC, respectively). After challenges, they were stored overnight in artificial saliva for a total of 24 hours and restored with Adper Single Bond 2/Filtek Z350. Buildup coronal surfaces were cut in 1 mm2-specimens and subjected to a microtensile test. Data were statistically analyzed by two-way ANOVA/Bonferroni tests (a=0.05). Failure modes were assessed by optical microscopy (X40). The Interface of the restorations were observed using Confocal Laser Scanning Microscopy (CLSM). Results: All tested cola-based drinks significantly reduced the bond strength, which was also observed in the analyses of interfaces. Toothbrushing did not have any impact on the bond strength. CLSM showed that except for Zero Coke, all eroded specimens resulted in irregular hybrid layer formation. Conclusions: All cola-based drinks reduced the bond strength. Different patterns of hybrid layers were obtained revealing their impact, except for ZC.
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