Recent Submissions

  • Frequency of exposure to Toxoplasma gondii in COVID-19 patients: a systematic review and meta-analysis

    León-Figueroa, Darwin A.; Barboza, Joshuan J.; Siddiq, Abdelmonem; Aguirre-Milachay, Edwin; Quispe-Vicuña, Carlos; Sah, Ranjit; Valladares-Garrido, Mario J.; Adhikari, Suraj; Bonilla-Aldana, D. Katterine; Ricardo-Martínez, Alex; Morales-Ramos, Jorge Guillermo; Rodriguez-Morales, Alfonso J. (BioMed Central Ltd, 2024-12-01)
    Background: Toxoplasmosis is a chronic protozoan parasitic infection that affects nearly one-third of the global population. During the COVID-19 pandemic, cases were observed in patients with COVID-19 and toxoplasmosis. Therefore, this systematic review and meta-analysis aimed to determine the frequency of Toxoplasma gondii exposure in patients with COVID-19. Methods: A literature search was conducted in six databases or search tools (PubMed, Scopus, Embase, Web of Science, ScienceDirect, and Google Scholar) until March 3, 2024. Study selection, quality assessment, and data extraction were performed independently by three investigators. Statistical analysis was performed using R version 4.3, applying a random-effects model. The quality of the included observational studies was assessed using the “JBI-MAStARI”. Results: A total of 5,936 studies were retrieved, 13 of which were included in the final meta-analysis. The sample included a total of 2,947 patients with COVID-19 from four countries, of whom approximately 43.3% were men and 49.4% were women. Among the patients, 1,323 showed evidence of exposure to T. gondii through IgG detection, while 1,302 COVID-19 patients were explicitly examined for T. gondii by IgM detection, and 36 positive cases were identified. The frequency of exposure to T. gondii, determined by the presence of IgG in patients with COVID-19, reached 49% (95% CI: 34–63%; 2,947 participants; 13 studies; I2 = 98%, p < 0.01). In addition, the frequency of exposure to T. gondii, evaluated by IgM presence in patients with COVID-19, was 2% (95% CI: 0–6%; 1,302 participants; 6 studies; I2 = 94%, p < 0.01). Conclusion: It was shown that almost half of COVID-19 patients had previous exposure to T. gondii through the presence of IgG, and a small percentage, 2%, showed active infection through IgM detection. Although the results indicate a possible correlation between exposure to T. gondii and the presence of COVID-19, it is essential to note that this study is based on observational research, which precludes establishing a causal relationship. Consequently, further research is required to deepen understanding of the interaction between the two conditions. Terms used: The Joanna Briggs Institute Meta-Analysis of Statistics Assessment and Review Instrument (JBI-MAStARI), Prospective International Registry of Systematic Reviews (PROSPERO), and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA).
    Acceso abierto
  • Laser gingivectomy for maintaining periodontal health in fixed orthodontic patients: a systematic review

    Zeballos, Blacker Kevin Caracela; Rubina, Yalina Nataly Alegre; Meza-Mauricio, Jonathan; Cafferata, Emilio A.; Vergara-Buenaventura, Andrea (Springer International Publishing, 2024-12-01)
    Objective: This systematic review evaluates the effect of laser gingivectomy on the improvement of periodontal health parameters in patients with gingival enlargement due to fixed orthodontic appliances. Materials and methods: A systematic review was conducted in 3 databases without language or date restrictions. Clinical trials involving patients with gingival enlargement (GE) or hyperplasia induced by fixed orthodontic treatment and treated by laser gingivectomy, conventional surgery (scalpel) or non-surgical periodontal treatment (NSPT) were included regarding periodontal parameters such as bleeding on probing, probing depth, gingival inflammation, among others. Results: From the 363 articles initially retrieved, only 6 were included after evaluation of the full text. However, all studies were considered to have a high risk of bias. It was detected that periodontal parameters (bleeding on probing, probing depth, gingival inflammation) were significantly lower in the laser-treated group when compared to NSPT. No statistically significant differences were observed between the use of laser or scalpel; however, the laser group offered faster healing and reduced postoperative complications. Conclusions: Within the limitations of this systematic review, the adjunctive use of laser gingivectomy to NSPT, for the treatment of GE induced by fixed orthodontic treatment offers favorable clinical results in the improvement of periodontal health parameters. In addition, it offers faster healing, less bleeding, less gingival swelling and postoperative pain, and better comfort for the patient compared with conventional surgery. Clinical relevance: The use of laser as an adjunct to NSPT proves to be a favorable and more comfortable option not only to treat GE but also to maintain periodontal health in patients with fixed orthodontics.
    Acceso restringido temporalmente
  • Psychometric network analysis of the Patient Health Questionnaire-4 (PHQ-4) in Paraguayan general population

    Caycho-Rodríguez, Tomás; Travezaño-Cabrera, Aaron; Torales, Julio; Barrios, Iván; Vilca, Lindsey W.; Samaniego-Pinho, Antonio; Moreta-Herrera, Rodrigo; Reyes-Bossio, Mario; Barria-Asenjo, Nicol A.; Ayala-Colqui, Jesús; Garcia-Cadena, Cirilo H. (Springer Science and Business Media Deutschland GmbH, 2024-12-01)
    Background: Depression and anxiety are two of the most prevalent and disabling mental disorders worldwide, both in the general population and in outpatient clinical settings. Objective: This study aimed to analyze the psychometric properties of the Patient Health Questionnaire-4 (PHQ-4) based on network analysis metrics. Methods: A total of 911 Paraguayans (23.71% women and 76.29% men; mean age 31.25 years, SD = 10.63), selected by non-probabilistic convenience sampling, participated in the study. Network analysis was used to evaluate the internal structure, reliability, and measurement invariance between men and women. Results: The results revealed that the PHQ-4 is a unidimensional measure through Exploratory Graph Analysis (EGA). Reliability, through structural consistency, identified that 100% of the time, only a single dimension was obtained, and all items remained stable, as they were always replicated within the empirical dimension. The unidimensional structure has shown evidence of configural invariance; therefore, the network structure functioned equally among the different sex groups. Conclusion: The PHQ-4 presented optimal preliminary evidence of validity based on its internal structure, reliability, and invariance between sexes. Therefore, it may be useful as an accurate and brief measure of anxiety and depressive symptoms in the Paraguayan context.
    Acceso abierto
  • Proposal to Reduce Transfer Time Through a Multimodal Interconnection Model

    Ramos Illaconza, Oscar Gianfranco; Cotos Caldas, Jeanpaul Vladimir; Lazo Benza, Omar Jesus (Springer Science and Business Media Deutschland GmbH, 2025-01-01)
    Transferring commuters on Atocongo (Lima, Perú), between Linea Amarilla (buses) and Line 1 of the Lima Metro (subway) shows various challenges. Commuters have to board the buses on the street, after crossing crowded roads, there are also interruptions like street vendors, crowded sidewalks, and lack of traffic signals at crosswalks. The transfer time is not optimized and the actios is not safe for commuters. This research proposes a multimodal interconnection that reduces transfer time and leads users directly between both transportation systems, on both routes: From Santiago de Surco (SS) and San Juan de Miraflores (SJM). The transfer time was obtained (current situation) and a model was developed with the interconnection infrastructure of both transport systems (proposed situation). From the comparison of both situations, it was obtained that the times were reduced, for the route from SS 49.87% and for the route from SJM 28.87%. It is concluded that the proposed model manages to reduce the user's transfering time.
    Acceso restringido temporalmente
  • A comparative analysis of heme vs non-heme iron administration: a systematic review and meta-analysis of randomized controlled trials

    Gallo Ruelas, Mariano; Alvarado-Gamarra, Giancarlo; Aramburu, Adolfo; Dolores-Maldonado, Gandy; Cueva, Karen; Rojas-Limache, Gabriela; Diaz-Parra, Carmen del Pilar; Lanata, Claudio F. (Springer Science and Business Media Deutschland GmbH, 2025-02-01)
    Background and purpose: Bioavailability studies and observational evidence suggest that heme iron (HI) may have greater impact on iron status indicators compared with non-heme iron (NHI). This systematic review and meta-analysis aimed to review the current evidence on the effect of the administration of HI compared with NHI for improving iron status in non-hospitalized population groups. Methods: We searched Pubmed, CENTRAL, Scopus, Web of Science, and LILACS from inception to July 2024. There was no language restriction or exclusion based on age or iron status. Only randomized controlled trials comparing HI with NHI were considered. A random-effects meta-analysis was performed to compare the effect of treatments for iron status indicators and total side effects (including gastrointestinal side effects). We measured the certainty of the evidence (CoE) using GRADE assessment. Results: After screening 3097 articles, 13 studies were included. Most of the interventions used HI in low doses combined with NHI. The meta-analysis showed higher hemoglobin increases in children with anemia or low iron stores receiving HI (MD 1.06 g/dL; 95% CI: 0.34; 1.78; CoE: very low). No statistically significant difference between interventions were found for any iron status indicator in the other population subgroups (CoE: very low). Participants receiving HI had a 38% relative risk reduction of total side effects compared to NHI (RR 0.62; 95% CI 0.40; 0.96; CoE: very low). Conclusion: The current evidence comparing HI with NHI is very limited, preliminary findings suggest that interventions using HI may result in fewer side effects and may be superior in children with iron deficiency or anemia. However, given the very low certainty of the evidence, these results need further investigation through high-quality clinical trials. Protocol registration: CRD42023483157.
    Acceso abierto
  • Adaptation, validation, and factorial invariance of a scale to assess the quality of virtual courses developed for Peruvian health sciences students during the COVID-19 pandemic

    Intimayta-Escalante, Claudio; Plasencia-Dueñas, Rubí; Nuñez-Lupaca, Janeth N.; Flores-Lovon, Kevin; Ramirez-Bernuy, Nancy; Pares-Ballasco, Giancarlo; Barrientos-Odias, Natalia; Castillo-Blanco, Ronald (Elsevier Espana S.L.U, 2025-05-01)
    Objective: To adapt, validate and evaluate the factorial invariance of a scale that assess the quality of virtual courses (QVC) among health sciences students (HSS). Methods: Cross-sectional study developed with virtual surveys to HSS to assess the QVC of the 2020 academic year, as well as sociodemographic and educational characteristics. Content-based validity was estimated with VAiken. Validity based on internal structure was assessed with the Goodness of Fit Indices (GFI) in the Confirmatory Factor Analysis (CFA), considering adequate the comparative fit index (CFI) and the Tucker-Lewis index (TLI) greater than 0.90, as well as values of the standardized root mean square residual (SRMR) and root mean square error of approximation (RMSEA) less than 0.08. Factor invariance was considered adequate when the ΔCFI ≤ 0.010 and the ΔRMSEA ≤ 0.015 between models at different levels. Reliability was assessed with the alpha and omega index. Results: Of the 629 HSS included, 62.96% were women, 49.76% studied human medicine and 316 other health areas. The VAiken was 0.86 on the instrument. The CFA showed adequate GFI (CFI = 0.956, TLI = 0.950, RMSEA = 0.073 and SRMR = 0.044). The ΔCFI (≤ 0.002) and ΔRMSEA (≤ 0.004) between models were estimated for the characteristics evaluated (sex, place of residence, study career, stage of training and type of university management). Reliability indices were greater than 0.85 in the domains. Conclusion: The 30-item scale and six domains to assess QVC developed for HSS in Peru showed adequate evidence of validity and reliability. In addition, it showed adequate factorial invariance in different sociodemographic and educational contexts of HSS.
    Acceso restringido temporalmente
  • Influence of Recycled Concrete and Ground Glass on the Compressive Strength of Concrete at 7 and 28 days

    Cordova, Jaime; Quispe, Marcell; Serrano, Malena (Springer Science and Business Media Deutschland GmbH, 2025-01-01)
    The main objective of the article is to assess the influence of using recycled aggregates on the compressive strength of concrete (f'c = 175 kg/cm2) at the age of 7 and 28 days. Specifically, the study focuses on the partial replacement of natural coarse and fine aggregates with recycled counterparts. To achieve this, recycled concrete from the demolition of columns in Villa el Salvador, Lima—Peru, was utilized as a substitute for the natural coarse aggregate. Additionally, ground glass sourced from glass shops in the Center of Lima was employed as a substitute for the natural fine aggregate. Three mixtures were designed following the ACI method: CC, representing conventional concrete; D1, denoting concrete with a 30% substitution (20% recycled concrete and 10% ground glass); and D2, indicating concrete with a 50% substitution (30% recycled concrete and 20% ground glass). Subsequent to the formulation of these mixtures, quality verification tests were conducted on the fresh concrete, and cylindrical specimens measuring 10 × 20 cm were created for sampling. After 7 days of curing, the specimens underwent compression tests, revealing strengths exceeding 65% of the design strength and after 28 days, strengths greater than 100% of the design strength.
    Acceso restringido temporalmente
  • Procedure to Improve Execution Speed Through Optimized Planning with Lob, Lps and Bim Methods in a Multifamily Building in Metropolitan Lima

    Pereda, Nicholas; Ríos, Jairo; Ulloa, Karem (Springer Science and Business Media Deutschland GmbH, 2025-01-01)
    As a developing country, Peru has great limitations in the application of new and innovative technologies in the construction sector. Construction projects consist of several stages in their development, one of the most important is the planning stage, which is essential to achieve efficient execution and meet established deadlines. However, this stage is usually tedious and complicated to carry out since there are multiple factors that can affect the estimates obtained from the planning process. However, the deadlines for the execution of construction projects are tight and failure to comply generates large losses of money. That is why this article seeks to implement the Lines of Balance (LOB), Last Planner System (LPS) and Building Information Modeling (BIM) methodologies to the traditional procedure. First, surveys were conducted on three different construction projects to determine the traditional planning process. Secondly, a procedure is developed to integrate the 3 aforementioned methodologies. Finally, the process was applied in a building project. A comparative analysis was carried out between the current state of the project and the state after using the aforementioned procedure. As a result, execution speeds increased for the items that maintained the same amount of work, the amount of m3 of slab emptying increased by 33.33%. It was observed that in the planning and work execution phase, the procedure improves the speed of execution of the items.
    Acceso restringido temporalmente
  • Evaluation of the Permeability of Concrete with the Addition of Glass Powder

    Josias Mikhail, Castillo Prado; José Sandro, Gomero Tello; Nayda Susana, Morales Galiano (Springer Science and Business Media Deutschland GmbH, 2025-01-01)
    The article presents the usefulness of implementing the use of recycled glass powder, after having carried out the permeability test, evaluated at the age of 28 days. This with the purpose of seeking a sustainable and low-cost alternative to improve the durability of concrete exposed to humid climates compared to conventional concrete. The influence of pulverized glass shows a positive impact on the mechanical properties of the concrete at the age of 28 days, through the permeability test. In a comparative study it is shown that adding glass powder produces workable samples obtained according to the slump test. On the one hand, being in a humid climate, water infiltration was measured due to the humidity of the concrete. For this purpose, the permeability test was carried out following what is described in the American standard ASTM C107. On the other hand, it was analyzed that powdered glass can improve the properties of concrete, since it has a positive contribution to the properties analyzed and achieving a waterproof concrete with a dosage of VP05. This brings benefits in the construction sector not only in improving the properties of concrete, but also in making more sustainable concretes.
    Acceso restringido temporalmente
  • Comparative Analysis of the Seismic Performance of a School with Metallic and Viscous Fluid Dampers in Lima, Peru.

    Loayza, Gabriela Alcantara; Hidalgo, Daniel Cristopher Solis; Huayra, Ruben Anccasi (Springer Science and Business Media Deutschland GmbH, 2025-01-01)
    The coastal zone of Peru is exposed to a high seismic risk, and many essential constructions in the country still lack seismic protection systems. This increases the likelihood of these structures experiencing considerable damage in the event of a severe earthquake, potentially leading to collapse. The present research proposes the implementation of Taylor-type viscous fluid dampers and Bozzo-type Shear Link metallic dampers, through two different designs applied to the same case study involving a reinforced concrete structure in the pavilion of a typical school built on soft ground in Lima. For this purpose, a spectral modal dynamic analysis of the building without dampers was conducted, as well as a linear time-history dynamic analysis of the structures with dampers. The results of these analyses provided the displacements and drifts of the structures. It was observed that the conventionally designed structure does not exhibit adequate structural behavior in the face of a severe earthquake, unlike the structures with damper implementation, which showed a significant reduction in their drifts and displacements. However, it was emphasized that the structure with the implementation of metallic dampers exhibited better structural behavior compared to the structure with viscous fluid dampers.
    Acceso restringido temporalmente
  • Comparative Study of Seismic Performance of SLB, TADAS and Viscous Fluid Energy Dissipators in Hospitals

    Bocanegra, Fabiana; Cervantes, Romina; Casas, Joan R.; Delgadillo, Rick M. (Springer Science and Business Media Deutschland GmbH, 2025-01-01)
    Structural damage not only causes material losses but also human losses, especially in the case of essential buildings such as health facilities, as they must ensure the provision of medical services during and after an earthquake. On the other hand, Peru is a highly seismic country due to its location on the western edge of South America, an area where the Nazca and South American tectonic plates converge. For this reason, it is essential to reinforce these infrastructures with devices that help prevent and reduce damage caused by earthquakes and guarantee the safety of the hospital. This paper studies the seismic performance of three energy dissipators: Shear Link Bozzo (SLB), Triangular Plate Added Damping and Stiffness (TADAS) and viscous fluid; incorporated into an existing hospital in Peru. The methodology involves modeling these three devices using ETABS software and evaluating the following study cases: (i) without energy dissipators, (ii) with the three seismic devices evaluated separately. Non-linear time-history analysis is conducted, considering seven seismic records compatible with the response spectrum constructed from the parameters of the Peruvian Standard E.030. Finally, it was concluded that the viscous fluid dissipator is the best option to implement in the hospital since the maximum drifts, accelerations and shear forces on each floor were reduced by 45, 23 and 27%.
    Acceso restringido temporalmente
  • Study of Internal Flow in a Liquid Nitrogen Flow Decelerator Through Swirl Effect Consisting of a Jet-Type Cryogenic Injection System for Food Freezing

    Arriaga, Ian; Sayán, Jasuo; Ronceros, Julio; Klusmann, Mirko; ALBATRINO, RENZO; Raymundo, Carlos; Zapata, Gianpierre; Ronceros, Gustavo (Multidisciplinary Digital Publishing Institute (MDPI), 2024-12-01)
    This article addresses the study of internal flow dynamics within a cryogenic chamber designed for freezing food using liquid nitrogen injection. The chamber features a circular section with strategically placed jet-type atomizers for this purpose. The primary objective is to extend the residence time of the cryogenic fluid within the chamber to ensure uniform and effective freezing of the passing food items. This is achieved by inducing a swirl effect through strategic deceleration of the flow using the atomizers. The meticulous placement of these atomizers at periodic intervals along the internal walls of the cylindrical chamber ensures prolonged recirculation of the internal flow. Internal temperature analysis is crucial to ensure the freezing process. The study is supported by numerical analysis in CFD ANSYS to assess the dynamics of the swirl effect and parameters associated with the nitrogen–air interface, from which we obtain a sophisticated analysis thanks to the design of a hexahedral mesh made in greater detail in ICEM CFD. This approach aims to understand internal flow behavior and its correlation with the complexity of cryogenic system design, utilizing variable nitrogen-injection pressures and strategic atomizer placement as fundamental parameters to optimize system design.
    Acceso restringido temporalmente
  • Correction to: Exploring effects of supplementary cementitious materials on setting time, strength, and microscale properties of mortar (Discover Applied Sciences, (2024), 6, 11, (583), 10.1007/s42452-024-06180-x)

    Romero, Lenin Miguel Bendezu; Bahrami, Alireza; Awoyera, Paul O.; Fadire, Oluwapamilerin; Velarde, Alan Yordan Valdivieso; Mydin, Md Azree Othuman; Arunachalam, Krishna Prakash (Springer Nature, 2024-12-01)
    In this article some of the authors’ details were incorrectly published, as listed below. The fifth author’s family name, Velarde, was incorrectly written as Valarde, and the author’s affiliation was incorrectly specified as ‘Department of Civil Engineering, Universidad Peruana de Ciencias Aplicadas, Lima, Peru’. It should be ‘Department of Civil Engineering, Universidad César Vallejo, Lima, Peru’. The fourth author’s affiliation was incorrectly specified as ‘Department of Civil Engineering, Prince Mohammad Bin Fahd University, Al Khobar, Saudi Arabia’. It should be ‘Department of Civil Engineering, Covenant University, Ota, Ogun State, Nigeria’. The last author’s email address ([email protected]) was missing. The original article has been corrected.
    Acceso abierto
  • Longitudinal transitions of the double burden of overweight and stunting from childhood to early adulthood in India, Peru, and Vietnam

    Escher, Nora A.; Carrillo-Larco, Rodrigo M.; Parnham, Jennie C.; Curi-Quinto, Katherine; Ghosh-Jerath, Suparna; Millett, Christopher; Seferidi, Paraskevi (Oxford University Press, 2024-12-01)
    Background: Examining trajectories of undernutrition and overnutrition separately limits understanding of the double burden of malnutrition. We investigated transitions between normal, stunting, overweight and concurrent stunting and overweight (CSO) and associations with sociodemographic factors in children and adolescents. Methods: We used data from the Young Lives cohort in India, Peru and Vietnam, which follow children 1-15 (N = 5413) and 8-22 years (N = 2225) over five rounds between 2002 and 2016. We estimated transitions between nutritional states using a Markov chain model and estimated sociodemographic associations employing a logit parametrization. Results: Transitions into stunting peaked in ages 1-5 years (India: 22.9%, Peru: 17.6%, Vietnam: 14.8%), while stunting reversal was highest during adolescence across all countries. Transitions into overweight peaked in ages 19-22, while overweight reversal increased in ages 1-5 and 12-15 years. Transitions away from stunting to overweight were rare; more commonly, stunted individuals developed overweight while remaining stunted, leading to a CSO state. In Peru, 20.2% of 19-year-olds who were stunted reached CSO by age 22, with 4% shifting from stunted to overweight. Reversion to a normal state is least likely for those in a CSO state. Household wealth gradually reduced the likelihood of transitioning into stunting [odds ratios (ORs) for wealthiest quartile in Peru: 0.29, 95% confidence interval (CI) 0.20-0.41; India: 0.43, 95% CI 0.32-0.57; Vietnam: 0.36, 95% CI 0.26-0.50), with stunting reversal only being more likely in the two wealthiest quartiles across all countries (ORs for wealthiest quartile in Peru: 2.39, 95% CI 1.57-3.65; India: 1.28, 95% CI 1.05-1.54; Vietnam: 1.89, 95% CI 1.23-2.91). In Vietnam, only the richest quartile was at higher risk of transitioning into overweight (OR 1.87, 95% CI 1.28-2.72), while in Peru and India, the risk gradually rose across all wealth quartiles (ORs for wealthiest quartile in Peru: 2.84, 95% CI 2.14-3.77; India: 2.99, 95% CI 1.61-5.54). Conclusions: Childhood and adolescence represent critical periods for prevention and reversal of stunting and overweight, thereby averting the development of CSO later in life. Context-specific interventions are crucial for preventing disparate transitions towards the double burden of malnutrition across socioeconomic groups.
    Acceso restringido temporalmente
  • Quantitative MODS-Wayne assay for rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis from sputum samples

    Toscano-Guerra, Emily; Alcántara, Roberto; Untiveros, Katherine Lozano; Gilman, Robert; Grandjean, Louis; Zimic, Mirko; Sheen, Patricia (American Society for Microbiology, 2024-12-01)
    Tuberculosis (TB) remains a significantglobal health challenge, exacerbated by the emergence of drug-resistant strains, such as those resistant to pyrazinamide (PZA). The current scarcity of affordableand precise quantitative diagnostic tests for PZA resistance underscores the urgent need for more accessible diagnostic tools. We evaluated PZA susceptibility in 264 TB-positive samples by quantifying pyrazinoic acid (POA) production, using both the MODS-Wayne qualitative assay and our newly developed quantitative approach (MODS-WQ). The MODS-WQ was assessed in 7H9 medium (MODS-WQ7H9) or citrate buffer(MODS-WQCB), with POA levels measured via spectrophotometry against a calibration curve. PZA susceptibility determinations were based on a composite reference standard. Associations between POA levels and pyrazinamidase mutations were explored. The composite standard detected PZA resistance in 23.5% of the samples, which accounts for 62.8% of the multidrug-resistant (MDR) samples. The MODS-WQ established specificPOA cutoffsof 123.25 μM for MODS-WQ7H9 and 664.7 μM for MODS-WQCB, with sensitivities of 81.3% and 92.3% and specificitiesof 77.2% and 95.9%, respectively. Notably, samples with mutations in the pyrazinamidase metal-binding site exhibited significantlylower POA levels compared with mutations in the enzyme periphery. Furthermore, a significantcorrelation was found between POA production and PZA resistance, Bactec Growth Index, and minimum inhibitory concentration (MIC) values. This study presents a novel, direct, and accessible susceptibility test for PZA resistance that quantifiesPOA, enhancing the detection capabilities for this condition. The citrate-bufferedMODS-WQ assay demonstrated high sensitivity and specificityfor quantifying POA, confirmingthat POA production is a reliable indicator of PZA resistance.
    Acceso restringido temporalmente
  • An in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of the split-GFP

    Quiroz-Huanca, Ana; Sanchez-Castro, Ana; Soriano-Castillo, Pablo; Poletti, Chiara; Nloh Tientcheu, Therese Manuela; Fabbretti, Attilio; Giuliodori, Anna Maria; Milon, Pohl (Cell Pres, 2024-12-20)
    Here, we present an in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of split-GFP for translation test (FAST), based on the small fragment GFP11 binding to GFP1-10fast. We detail the expression and purification of the GFP1-10fast protein, DNA template amplification, in vitro GFP11-tagged CspA synthesis, FAST detection of the GFP11-tagged protein, and optional recovery of the fluorescent complex. In vitro synthesis of GFP11 maximizes the molar yield of synthesized proteins, providing enhanced sensitivity to test translation inhibitors. For complete details on the use and execution of this protocol, please refer to Pham et al.
    Acceso restringido temporalmente
  • Avances sobre ergonomia en tareas de paletizacion

    Escobar Galindo, Carlos Manuel; Mendoza Correa, María del Milagro (2014-12-31)
    Objetivo: El siguiente estudio de revisión bibliográfica se propone como objetivo general evidenciar con bibliografía actualizada, estudios que nos permitan comprender la magnitud del riesgo que afronta el trabajador frente a las demandas que exige las tareas de paletizado, a nivel biomecánico, fisiológico y riesgos relacionados al trabajo así como el beneficio de la implementación de apoyos mecánicos. Material y Métodos: Se realizo una búsqueda bibliográfica en los diferentes motores de búsqueda: Google académico, HINARI, revista APPLIED ERGONOMICS de editorial Elsevier y revista ERGONOMICS de Taylor & Francis, así mismo también se usaron textos de consultas actuales sobre ergonomía y biomecánica. Resultados: Se evidencian que la carga física biomecánica al paletizar es alta sobretodo en trabajos donde los trabajadores no tienen experiencia, así mismo la forma de la caja, el tamaño, la distancia de paletizado, la frecuencia y el modo operatorio afecta notablemente el riesgo de lesión en columna lumbosacra así como incrementa el nivel de gasto energético. Se adoptan los criterios establecidos por NIOSH (1994) que establecen no sobrepasar el 50% de la capacidad aeróbica en tareas que duren menos o una hora, el 40 % en tareas que duren entre 1 y 2 horas y el 33% en tareas que duren entre 2 a 8 horas. Conclusiones: Las técnicas posturales empleadas por trabajadores experimentados no garantizan una protección elevada contra el riesgo de lesión de columna, sin embargo las técnicas son mejores y mas estables para disminuir el riesgo que en los trabajadores no experimentados. El costo beneficio es un factor importante al momento de buscar soluciones para justificar el gasto, en el estudio presentado sobre grúas en pacientes se observa que se ahorra hasta en 70% en costos directos e indirectos al trabajador.
    Acceso abierto
  • Un análisis de la relación entre la racionalidad y la ética alrededor de la toma de decisiones en las organizaciones

    Ojeda Pino, Jorge Luis (Universidad Peruana de Ciencias Aplicadas, 2020-06-26)
    El proceso decisorio es un elemento clave en diversos campos. La racionalidad es un marco teórico para entender y formular el comportamiento social y económico que contribuye a la búsqueda de mejores decisiones. Este rol de la racionalidad genera diversas expectativas de parte de quienes interactúan con el proceso decisorio; una de estas expectativas es que debe incorporar criterios éticos en el proceso decisorio. Esta reflexión explora, tanto el concepto de racionalidad, como sus tipos, a fin de evaluar qué tanto debería o no incorporar estos criterios éticos. La exploración incluye los conceptos de racionalidad instrumental mínima y ética, proponiendo un análisis de sus puntos de contacto y de sus roles en cuanto al conocimiento previo a la toma de decisiones, el razonamiento, las acciones derivadas del proceso decisorio y sus consecuencias. Se concluye que la racionalidad y la ética comparten diversos campos de implementación del proceso decisorio, pero cada una desde su propio rol
    Acceso abierto
  • La gerencia interpelada: educación en gestión versus formación en valores

    Peña Cavassa, Viviana H. (2018-07-23)
    El caso Lava Jato ha desatado el, probablemente, mayor escándalo empresarial que la historia haya conocido. Una trama escalofriante, cuyo esclarecimiento está aún en proceso. Apenas conocemos la punta del iceberg. Sin embargo, los primeros detalles revelan una contundente realidad: la corrupción instalada en el seno de nuestra sociedad y en sus instituciones. ¿Qué nos ello dice del carácter de la gestión?
    Acceso abierto
  • Bending Response of Doubly Curved Laminated Composite Shells using Hybrid Refined Models

    Monge, J; Mantari, J; Yarasca, J; Arciniega, R (IOP Publishing, 2019-02-26)
    This study presents a static analysis of laminated composite doubly curved shells using a refined kinematic model with polynomial and non-polynomial functions. In particular Maclaurin, trigonometric, exponential and zig-zag functions are employed. Refined models are based on the Equivalent Single Layer theories and obtained by using Carrera Unified formulation. The shell model is subjected to different mechanical loading such as bi-sinusoidal, uniform and point load. The governing equations are derived from the principle of virtual displacement and solved via Navier-Type closed form solutions. The results are compared with Layer-wise and higher-order shear deformation solutions available in the literature. It is shown that refined models with non-polynomial terms are capable of accurately predicting the through-the-thickness displacements and stress distributions with a low computational effort.
    Acceso abierto

View more