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Efecto antibacteriano y citotóxico de dos extractos metanólicos a base de Moringa oleifera (moringa) y Azadirachta indica (neem) sobre cepas de Enterococcus faecalis

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Authors
Arévalo Híjar, Olga Lucía
Advisors
Gonzáles Soto, Néstor
Del Valle Mendoza, Juana
Issue Date
22/02/2017
Keywords
Extractos metanólicos
Antibacteriano
Odontología

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Citation
1. Híjar A, Lucía O. Efecto antibacteriano y citotóxico de dos extractos metanólicos a base de Moringa oleifera ( moringa ) y Azadirachta indica ( neem ) sobre cepas de Enterococcus faecalis [Internet]. Universidad Peruana de Ciencias Aplicadas (UPC); 2017. Available from: http://hdl.handle.net/10757/621020
Publisher
Universidad Peruana de Ciencias Aplicadas (UPC)
URI
http://hdl.handle.net/10757/621020
Abstract
Objetivo: Evaluar in vitro el efecto antibacteriano y citotóxico de dos extractos metanólicos de Azadirachta indica y Moringa oleifera sobre cepas de Enterococcus faecalis (ATCC 29212). Métodos: Los extractos metanólicos de Azadirachta indica y Moringa oleifera fueron preparados in vitro. El efecto antibacteriano de los extractos frente a cepas de Enterococcus faecalis fueron evaluados por medio de la técnica de difusión en agar (perforación en gel). La concentración mínima inhibitoria (CMI) se determinó por el método de microdilución y la citotoxicidad usando la línea celular MDCK. Resultados: El extracto metanólico que obtuvo mayor efecto antibacteriano en 24 y 48 horas frente al Enterococcus faecalis fue la Moringa oleifera, obteniendo un halo de 35.5 ± 1.05 y 44.83 ± 0.98, respectivamente. La CMI para ambos extractos fue de 75 µg/ml. El efecto bactericida para el extracto de Azadirachta indica fue a concentración de 25 µg/ml y para el extracto de Moringa oleífera fue de 75 µg/ml. Conclusiones: Se demostró que los extractos metanólicos de Azadirachta indica y Moringa oleifera tienen efecto antibacteriano contra cepas de Enterococcus faecalis a las 24 y 48 horas. Ninguno de los dos extractos resultó ser tóxico sobre líneas celulares a bajas concentraciones.
Objective: To evaluate in vitro the antibacterial and cytotoxic effect of two methanolic extracts of Azadirachta indica and Moringa oleifera on strains of Enterococcus faecalis (ATCC 29212). Methods: The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against strains of Enterococcus faecalis were evaluated by agar diffusion technique (gel perforation). Minimum inhibitory concentration (MIC) was determined by the microdilution method and cytotoxicity using the MDCK cell line. Results: The methanolic extract that obtained the highest antibacterial effect in 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, obtaining a halo of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 μg / ml. The bactericidal effect for Azadirachta indica extract was 25 μg / ml and for Moringa oleifera extract it was 75 μg / ml. Conclusions: It was demonstrated that the methanolic extracts of Azadirachta indica and Moringa oleifera have an antibacterial effect against strains of Enterococcus faecalis at 24 and 48 hours. Neither extract was toxic to cell lines at low concentrations.
Type
info:eu-repo/semantics/bachelorThesis
Rights
info:eu-repo/semantics/embargoedAccess
Language
spa
Collections
Odontologia

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