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Diagnosis of Carrion’s Disease by Direct Blood PCR in Thin Blood Smear Negative Samples.

dc.contributor.authorDel Valle Mendoza, Juana*
dc.contributor.authorSilva-Caso, Wilmer*
dc.contributor.authorTinco Valdez, Carmen*
dc.contributor.authorPons, Maria J.*
dc.contributor.authorDel Valle, Luis J.*
dc.contributor.authorCasabona Oré, Verónica*
dc.contributor.authorChampin Michelena, Denisse*
dc.contributor.authorBazán Mayra, Jorge*
dc.contributor.authorZavaleta Gavidea, Víctor*
dc.contributor.authorVargas, Martha*
dc.contributor.authorRuiz, Joaquim*
dc.date.accessioned2014-04-12T01:53:53Z
dc.date.available2014-04-12T01:53:53Z
dc.date.issued2014-04-11
dc.identifier.citationPLoS ONE 9(3): e92283es_PE
dc.identifier.doi10.1371/journal.pone.0092283
dc.identifier.urihttp://hdl.handle.net/10757/315714es_PE
dc.descriptionBartonella bacilliformis is the etiologic agent of Carrion’s disease. This disease has two well established phases, the most relevant being the so called Oroya Fever, in which B. bacilliformis infect the erythrocytes resulting in severe anemia and transient immunosuppression, with a high lethality in the absence of adequate antibiotic treatment. The presence of B. bacilliformis was studied in 113 blood samples suspected of Carrion’s disease based on clinical criteria, despite the absence of a positive thin blood smear, by two different PCR techniques (using Bartonella-specific and universal 16S rRNA gene primers), and by bacterial culture. The specific 16S rRNA gene primers revealed the presence of 21 B. bacilliformis and 1 Bartonella elizabethae, while universal primers showed both the presence of 3 coinfections in which a concomitant pathogen was detected plus Bartonella, in addition to the presence of infections by other microorganisms such as Agrobacterium or Bacillus firmus. These data support the need to implement molecular tools to diagnose Carrion’s disease.es_PE
dc.description.sponsorshipThis work has been partially supported by Optimus Foundation and by personal funds of JdVM. JR is supported by the program I3, of the ISCIII (grant number: CES11/012) and by ISCIII grant PI11/00983. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherPublic Library of Science (PLoS)es_PE
dc.relation.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0092283es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.titleDiagnosis of Carrion’s Disease by Direct Blood PCR in Thin Blood Smear Negative Samples.es_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.eissn1932-6203
dc.identifier.journalPLoS ONEes_PE
dc.description.peer-reviewRevisión por pares.es_PE
refterms.dateFOA2018-06-19T09:36:15Z


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