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Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

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Authors
Weilg Espejo, Pablo
Orellana Peralta, Fiorella
Cornejo Pacheres, Hernán
Del Valle, Luis J.
Cornejo Tapia, Ángela
Bazán Mayra, Jorge
Ruiz, Joaquim
Del Valle Mendoza, Juana
Issue Date
2014-03-10
Keywords
Viral genotypes
Epidemiology
Acute gastroenteritis
Peru

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Citation
Trans R Soc Trop Med Hyg first published online April 27, 2014. doi:10.1093/trstmh/tru059
Publisher
Oxford University Press
URI
http://hdl.handle.net/10757/313901
DOI
10.1093/trstmh/tru059
Additional Links
http://www.ncbi.nlm.nih.gov/pubmed/24778205
Abstract
Background: Gastroenteritis by rotavirus is responsible for approximately 810 annual deaths/year in children under 5 years in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular-epidemiology of rotavirus diarrhea in Peru Methodology: A total of 131 stool samples were obtained from children under 5 years old hospitalized from January 2010 to December 2012 in the Hospital Regional de Cajamarca, Peru. ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were obtained by semi-nested multiplex RT-PCR and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 18.80% (22/117) tested positive for rotavirus by ELISA and 35.90% (42/117) by RT-PCR. Among the G-genotype identified, G9 in 35.71% (15/42) and G12 in 33.33% (14/42) were the most prevalent. With the most common combination being G12/P6 in 23.81% (10/42). Conclusions: A high prevalence of the G12/P6 genotype was detected. It is know that this genotype is not covered by the current vaccines available. More in depth studies are needed to know the current rotavirus genotypes presents in Peru.
Type
info:eu-repo/semantics/article
Rights
info:eu-repo/semantics/openAccess
Language
eng
Description
Artículo sustentado el 30 de Enero 2014 para la obtención del título profesional Médico Cirujano en la Escuela de Medicina, Facultad de Ciencias de la Salud. Universidad Peruana de Ciencias Aplicadas - UPC.
Artículo publicado el 27 de Abril de 2014 en la Revista Transactions of the Royal Society of Tropical Medicine & Hygiene (Oxford University Press).
ISSN
1878-3503
EISSN
0035-9203
ae974a485f413a2113503eed53cd6c53
10.1093/trstmh/tru059
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Medicina

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