In vitro evaluation of bacterial adhesion and bacterial viability of Streptococcus mutans, Streptococcus sanguinis, Porphyromonas gingivalis and the abutment surface of the dental implants of titanium and zirconium
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Autor
Meza, Ana StefanyAsesores
Del Valle Mendoza, Juana MercedesFecha de publicación
2017-11-22
Metadatos
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Juana, D., Del, M., & Mendoza, V. (2017). In vitro evaluation of bacterial adhesion and bacterial viability of Streptococcus mutans , Streptococcus sanguinis , Porphyromonas gingivalis and the abutment surface of the dental implants of titanium and zirconium. Universidad Peruana de Ciencias Aplicadas (UPC). Retrieved from http://hdl.handle.net/10757/622856Resumen
Objetivo: Evaluar in vitro la adherencia y viabilidad bacteriana de Streptococcus mutans, Streptococcus sanguinis y Porphyromonas gingivalis en la superficie del abutment de los implantes de titanio y zirconio. Métodos: Se prepararon seis muestras, divididas en dos grupos, G1: 3 abutment de titanio y G2: 3 abutment de zirconio con tornillos de fijación, cada grupo fue embebido en tubos con cultivos bacterianos, posteriormente las muestras se incubaron a 37 ° C en condiciones de anaerobiosis. La adherencia bacteriana se evaluo en UFC y la viabilidad bacteriana con prueba colorimétrica MTT. Resultados: Se constató que el abutment de titanio tiene mayor adherencia de S. mutans (190.90 UFC / ml), mientras que P. gingivalis muestra mayor viabilidad bacteriana (73.22%). Para el abutment de zirconio se observó mayor adhesión, para el caso de S. mutans (331.82 UFC / ml) y mayor viabilidad en la cepa de S. sanguinis (38.42%). En los tornillos de fijación, en el titanio se observó que la mayor adherencia para el caso de S. sanguinis (132.5 UFC / ml) y mayor viabilidad para S. mutantes (78.04%). Mientras que para el tornillo de zirconio se observa que S. mutans tiene mayor adherencia y viabilidad con (145.5 UFC / ml) y ( 57.38%) respectivamente. Conclusiones: Existe mayor adherencia bacteriana en los abutment de zirconio, sin embargo, la viabilidad bacteriana es menor. Mientras, para el caso del abutment de titanio se encontró que existe menor adherencia pero mayor viabilidad bacteriana. Se sugiere ampliar con los estudiantes que tienen mayor número de muestra.Objective: To evaluate in vitro the adherence and bacterial viability of Streptococcus mutans, Streptococcus sanguinis and Porphyromonas gingivalis on the abutment surface of titanium and zirconium implants. Methods: Six samples were prepared, divided into two groups, G1: 3 abutment of titanium and G2: 3 abutment of zirconium with their fixation screws, each group was embedded in tubes with bacterial cultures, subsequently the samples were incubated at 37 ° C in conditions of anaerobiosis. Bacterial adherence was evaluated in CFU and bacterial viability with the MTT colorimetric test. Results: It was found that in the titanium abutment there is greater adherence of S. mutans (190.90 CFU / ml), while P. gingivalis shows greater bacterial viability (73.22%). For the zirconia abutment, greater adhesion was observed, for the case of S. mutans (331.82 CFU / ml) and greater viability in the S. sanguinis strain (38.42%). In the fixing screws, titanium showed the highest adherence for S. sanguinis (132.5 CFU / ml) and greater viability for S. mutants (78.04%). While for the zirconium screw it is observed that S. mutans has greater adherence and viability with (145.5 CFU / ml) and (57.38%) respectively. Conclusions: There is greater bacterial adherence in zirconium abutment, however, bacterial viability is lower. Meanwhile, in the case of titanium abutment it was found that there is less adherence but greater bacterial viability. It is suggested to expand with students who have a larger sample number.
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