Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections
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Authors
Del Valle, Luis J.Jaramillo, Michael L.
Talledo, Miguel
Pons, Maria J.
Flores, Lidia
Quispe, Ruth L.
Ramírez, Pablo
García de la Guarda, Ruth
Alvarado, Débora
Espinoza-Culupú, Abraham
Del Valle Mendoza, Juana
Vargas, Martha
Ruíz, Joaquim
Issue Date
2014-07-02
Metadata
Show full item recordPublisher
Horizon Research PublishingJournal
Universal Journal of Microbiology ResearchDOI
10.13189/ujmr.2014.020102Additional Links
http://www.hrpub.org/download/20131215/UJMR2-10201796.pdfAbstract
Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.Type
info:eu-repo/semantics/articleRights
info:eu-repo/semantics/openAccessLanguage
engISSN
2331-6721EISSN
2331-673Xae974a485f413a2113503eed53cd6c53
10.13189/ujmr.2014.020102
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