Phytochemical Screening, Total Phenolic Content, Antioxidant and Cytotoxic Activity of Chromolaena laevigata on Human Tumor Cell Lines

2.50
Hdl Handle:
http://hdl.handle.net/10757/622501
Title:
Phytochemical Screening, Total Phenolic Content, Antioxidant and Cytotoxic Activity of Chromolaena laevigata on Human Tumor Cell Lines
Authors:
Herrera-Calderon, Oscar; Arroyo-Acevedo, Jorge; Rojas-Armas, Juan; Chumpitaz-Cerrate, Victor; Figueroa-Salvador, Linder; Enciso-Roca, Edwin; Tinco-Jayo, Johnny
Citation:
Phytochemical Screening, Total Phenolic Content, Antioxidant and Cytotoxic Activity of Chromolaena laevigata on Human Tumor Cell Lines 2017, 21 (1):1 Annual Research & Review in Biology
Publisher:
SCIENCEDOMAIN international
Journal:
Annual Research & Review in Biology
Issue Date:
16-Dec-2017
URI:
http://hdl.handle.net/10757/622501
DOI:
10.9734/ARRB/2017/37045
Additional Links:
http://www.sciencedomain.org/abstract/22330
Abstract:
Aims: Cancer is the first cause of death in the Peruvian population; searching alternative treatments of medicinal plants constitute a promissory field to find new anticancer drugs. The main objective in this study was to evaluate the phytochemical screening, total phenolic content, antioxidant and cytotoxic activity of ethanol extract of Chromolaena laevigata (C. laevigata) on human tumor cell lines. Study Design: The fresh leaves of C. laevigata were soaked with ethanol followed by phytochemical screening using standard methods. Place and Duration of Study: Laboratory of Applied Chemistry, Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica, Ica, Peru; Laboratory “Abraham Vaisberg Wolach”, Universidad Peruana Cayetano Heredia, Lima, Peru. Methodology: Phytochemical screening was assessed by using chemical reactives. Total phenolic content (TPC) was developed using Folin Ciocalteu reactive and the antioxidant activity was determined against DPPH and ABTS radicals by spectrophotometry. The cytotoxic activity was determined on human tumor cell lines followed as: MCF-7, H-460, HT-29, M-14, K-562 and DU-145. Results: Phytochemical study confirmed flavonoids and phenolic compounds in ethanol extract. TPC resulted 45.21 ± 3.5 mg of gallic acid equivalent/g of dried extract. The highest antioxidant extract for DPPH and ABTS radical scavenging tests were IC50 = 11.66 ± 1.0 μg/mL, IC50= 12.45 ± 0.50 μg/mL respectively. Ethanolic extracts (μg/mL) showed a low cytotoxicity on human tumor cell lines (CI50 > 20 μg/mL) for DU-145, HT-29, MCF-7 and M-14. Whereas, for H-460, and K562 tumor cell lines showed high cytotoxicity. Conclusion: In our findings, C. laevigata demonstrated a high antioxidant and total phenolic content. The ethanol extract exhibited better cytotoxic effect compared with 5-FU. Hence, This medicinal plant could be effective to prevent chronical diseases as cancer and oxidative stress disorders.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Anticancer; Antioxidants; Chromolaena laevigata; Cytotoxicity; Flavonoids; Oxidative stress; Phytochemical
ISSN:
2347565X

Full metadata record

DC FieldValue Language
dc.contributor.authorHerrera-Calderon, Oscares
dc.contributor.authorArroyo-Acevedo, Jorgees
dc.contributor.authorRojas-Armas, Juanes
dc.contributor.authorChumpitaz-Cerrate, Victores
dc.contributor.authorFigueroa-Salvador, Linderes
dc.contributor.authorEnciso-Roca, Edwines
dc.contributor.authorTinco-Jayo, Johnnyes
dc.date.accessioned2018-01-09T16:45:21Z-
dc.date.available2018-01-09T16:45:21Z-
dc.date.issued2017-12-16-
dc.identifier.citationPhytochemical Screening, Total Phenolic Content, Antioxidant and Cytotoxic Activity of Chromolaena laevigata on Human Tumor Cell Lines 2017, 21 (1):1 Annual Research & Review in Biologyes
dc.identifier.issn2347565X-
dc.identifier.doi10.9734/ARRB/2017/37045-
dc.identifier.urihttp://hdl.handle.net/10757/622501-
dc.description.abstractAims: Cancer is the first cause of death in the Peruvian population; searching alternative treatments of medicinal plants constitute a promissory field to find new anticancer drugs. The main objective in this study was to evaluate the phytochemical screening, total phenolic content, antioxidant and cytotoxic activity of ethanol extract of Chromolaena laevigata (C. laevigata) on human tumor cell lines. Study Design: The fresh leaves of C. laevigata were soaked with ethanol followed by phytochemical screening using standard methods. Place and Duration of Study: Laboratory of Applied Chemistry, Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica, Ica, Peru; Laboratory “Abraham Vaisberg Wolach”, Universidad Peruana Cayetano Heredia, Lima, Peru. Methodology: Phytochemical screening was assessed by using chemical reactives. Total phenolic content (TPC) was developed using Folin Ciocalteu reactive and the antioxidant activity was determined against DPPH and ABTS radicals by spectrophotometry. The cytotoxic activity was determined on human tumor cell lines followed as: MCF-7, H-460, HT-29, M-14, K-562 and DU-145. Results: Phytochemical study confirmed flavonoids and phenolic compounds in ethanol extract. TPC resulted 45.21 ± 3.5 mg of gallic acid equivalent/g of dried extract. The highest antioxidant extract for DPPH and ABTS radical scavenging tests were IC50 = 11.66 ± 1.0 μg/mL, IC50= 12.45 ± 0.50 μg/mL respectively. Ethanolic extracts (μg/mL) showed a low cytotoxicity on human tumor cell lines (CI50 > 20 μg/mL) for DU-145, HT-29, MCF-7 and M-14. Whereas, for H-460, and K562 tumor cell lines showed high cytotoxicity. Conclusion: In our findings, C. laevigata demonstrated a high antioxidant and total phenolic content. The ethanol extract exhibited better cytotoxic effect compared with 5-FU. Hence, This medicinal plant could be effective to prevent chronical diseases as cancer and oxidative stress disorders.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherSCIENCEDOMAIN internationales
dc.relation.urlhttp://www.sciencedomain.org/abstract/22330es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAnticanceres
dc.subjectAntioxidantses
dc.subjectChromolaena laevigataes
dc.subjectCytotoxicityes
dc.subjectFlavonoidses
dc.subjectOxidative stresses
dc.subjectPhytochemicales
dc.titlePhytochemical Screening, Total Phenolic Content, Antioxidant and Cytotoxic Activity of Chromolaena laevigata on Human Tumor Cell Lineses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.journalAnnual Research & Review in Biologyes
dc.description.peerreviewRevisión por pareses_PE
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