Immunosuppressive and angiogenic cytokine profile associated with Bartonella bacilliformis infection in post-outbreak and endemic areas of Carrion's disease in Peru

2.50
Hdl Handle:
http://hdl.handle.net/10757/622210
Title:
Immunosuppressive and angiogenic cytokine profile associated with Bartonella bacilliformis infection in post-outbreak and endemic areas of Carrion's disease in Peru
Authors:
Pons, Maria J.; Gomes, Cláudia; Aguilar, Ruth ( 0000-0003-3277-3107 ) ; Barrios, Diana; Aguilar-Luis, Miguel Angel; Ruiz, Joaquim ( 0000-0002-4431-2036 ) ; Dobaño, Carlota; del Valle-Mendoza, Juana; Moncunill, Gemma ( 0000-0001-5105-9836 )
Citation:
Immunosuppressive and angiogenic cytokine profile associated with Bartonella bacilliformis infection in post-outbreak and endemic areas of Carrion's disease in Peru 2017, 11 (6):e0005684 PLOS Neglected Tropical Diseases
Publisher:
Public Library of Science
Journal:
PLOS Neglected Tropical Diseases
Issue Date:
19-Jun-2017
URI:
http://hdl.handle.net/10757/622210
DOI:
10.1371/journal.pntd.0005684
Additional Links:
http://dx.plos.org/10.1371/journal.pntd.0005684
Abstract:
Analysis of immune responses in Bartonella bacilliformis carriers are needed to understand acquisition of immunity to Carrion’s disease and may allow identifying biomarkers associated with bacterial infection and disease phases. Serum samples from 144 healthy subjects from 5 villages in the North of Peru collected in 2014 were analyzed. Four villages had a Carrion’s disease outbreak in 2013, and the other is a traditionally endemic area. Thirty cytokines, chemokines and growth factors were determined in sera by fluorescent bead-based quantitative suspension array technology, and analyzed in relation to available data on bacteremia quantified by RT-PCR, and IgM and IgG levels measured by ELISA against B. bacilliformis lysates. The presence of bacteremia was associated with low concentrations of HGF (p = 0.005), IL-15 (p = 0.002), IL-6 (p = 0.05), IP-10 (p = 0.008), MIG (p = 0.03) and MIP-1α (p = 0.03). In multi-marker analysis, the same and further TH1-related and pro-inflammatory biomarkers were inversely associated with infection, whereas angiogenic chemokines and IL-10 were positively associated. Only EGF and eotaxin showed a moderate positive correlation with bacteremia. IgM seropositivity, which reflects a recent acute infection, was associated with lower levels of eotaxin (p = 0.05), IL-6 (p = 0.001), and VEGF (p = 0.03). Only GM-CSF and IL-10 concentrations were positively associated with higher levels of IgM (p = 0.01 and p = 0.007). Additionally, IgG seropositivity and levels were associated with high levels of angiogenic markers VEGF (p = 0.047) and eotaxin (p = 0.006), respectively. Our findings suggest that B. bacilliformis infection causes immunosuppression, led in part by overproduction of IL-10. This immunosuppression probably contributes to the chronicity of asymptomatic infections favoring B. bacilliformis persistence in the host, allowing the subsequent transmission to the vector. In addition, angiogenic markers associated with bacteremia and IgG levels may be related to the induction of endothelial cell proliferation in cutaneous lesions during chronic infections, being possible candidate biomarkers of asymptomatic infections.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Bacteremia; Biomarkers; Reverse transcriptase-polymerase chain reaction; Immune response; Cytokines; Chemokines; Bartonella; Endothelial cells
ISSN:
1935-2735
Email:
jdelvall@upc.edu.pe

Full metadata record

DC FieldValue Language
dc.contributor.authorPons, Maria J.es
dc.contributor.authorGomes, Cláudiaes
dc.contributor.authorAguilar, Ruthes
dc.contributor.authorBarrios, Dianaes
dc.contributor.authorAguilar-Luis, Miguel Angeles
dc.contributor.authorRuiz, Joaquimes
dc.contributor.authorDobaño, Carlotaes
dc.contributor.authordel Valle-Mendoza, Juanaes
dc.contributor.authorMoncunill, Gemmaes
dc.date.accessioned2017-10-09T17:11:19Z-
dc.date.available2017-10-09T17:11:19Z-
dc.date.issued2017-06-19-
dc.identifier.citationImmunosuppressive and angiogenic cytokine profile associated with Bartonella bacilliformis infection in post-outbreak and endemic areas of Carrion's disease in Peru 2017, 11 (6):e0005684 PLOS Neglected Tropical Diseaseses
dc.identifier.issn1935-2735-
dc.identifier.doi10.1371/journal.pntd.0005684-
dc.identifier.urihttp://hdl.handle.net/10757/622210-
dc.description.abstractAnalysis of immune responses in Bartonella bacilliformis carriers are needed to understand acquisition of immunity to Carrion’s disease and may allow identifying biomarkers associated with bacterial infection and disease phases. Serum samples from 144 healthy subjects from 5 villages in the North of Peru collected in 2014 were analyzed. Four villages had a Carrion’s disease outbreak in 2013, and the other is a traditionally endemic area. Thirty cytokines, chemokines and growth factors were determined in sera by fluorescent bead-based quantitative suspension array technology, and analyzed in relation to available data on bacteremia quantified by RT-PCR, and IgM and IgG levels measured by ELISA against B. bacilliformis lysates. The presence of bacteremia was associated with low concentrations of HGF (p = 0.005), IL-15 (p = 0.002), IL-6 (p = 0.05), IP-10 (p = 0.008), MIG (p = 0.03) and MIP-1α (p = 0.03). In multi-marker analysis, the same and further TH1-related and pro-inflammatory biomarkers were inversely associated with infection, whereas angiogenic chemokines and IL-10 were positively associated. Only EGF and eotaxin showed a moderate positive correlation with bacteremia. IgM seropositivity, which reflects a recent acute infection, was associated with lower levels of eotaxin (p = 0.05), IL-6 (p = 0.001), and VEGF (p = 0.03). Only GM-CSF and IL-10 concentrations were positively associated with higher levels of IgM (p = 0.01 and p = 0.007). Additionally, IgG seropositivity and levels were associated with high levels of angiogenic markers VEGF (p = 0.047) and eotaxin (p = 0.006), respectively. Our findings suggest that B. bacilliformis infection causes immunosuppression, led in part by overproduction of IL-10. This immunosuppression probably contributes to the chronicity of asymptomatic infections favoring B. bacilliformis persistence in the host, allowing the subsequent transmission to the vector. In addition, angiogenic markers associated with bacteremia and IgG levels may be related to the induction of endothelial cell proliferation in cutaneous lesions during chronic infections, being possible candidate biomarkers of asymptomatic infections.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pntd.0005684es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectBacteremiaes
dc.subjectBiomarkerses
dc.subjectReverse transcriptase-polymerase chain reactiones
dc.subjectImmune responsees
dc.subjectCytokineses
dc.subjectChemokineses
dc.subjectBartonellaes
dc.subjectEndothelial cellses
dc.titleImmunosuppressive and angiogenic cytokine profile associated with Bartonella bacilliformis infection in post-outbreak and endemic areas of Carrion's disease in Perues
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.journalPLOS Neglected Tropical Diseaseses
dc.description.fundingThis work was supported by Cienciativa of CONCYTEC Peru, under the contract N° 193-2015-FONDECYT, and the Agència de Gestió d’Ajuts Universitaris i de Recerca AGAUR [2014SGR991]. CG had a PhD fellowship from the ISCIII (FI12/00561). JR had a fellowship from the I3 program of the ISCIII [grant number: CES11/012]. MJP had a fellowship from the Programa de movilizacion internacional CTel (010-2015-CONCYTEC-P). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Volunteers of Piura Department. Field, Clinical and lab personnel. Hector Sanz and Itziar Ubillos for statistical advice. ISGlobal is a member of the CERCA Programme, Generalitat de Catalunya.es_PE
dc.description.peerreviewRevisión por pareses_PE
dc.contributor.emailjdelvall@upc.edu.pees_PE
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