Detección y genotipificación de rotavirus en pacientes con gastroenteritis aguda

4.00
Hdl Handle:
http://hdl.handle.net/10757/620965
Title:
Detección y genotipificación de rotavirus en pacientes con gastroenteritis aguda
Authors:
Weilg Espejo, Pablo
Advisors:
Del Valle Mendoza, Juana Mercedes ( 0000-0002-6011-5040 )
Publisher:
Universidad Peruana de Ciencias Aplicadas (UPC)
Issue Date:
30
URI:
http://hdl.handle.net/10757/620965
Additional Links:
http://hdl.handle.net/10757/313901
Abstract:
Background: Gastroenteritis by rotavirus is responsible for approximately 810 annual deaths/year in children under 5 years in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular-epidemiology of rotavirus diarrhea in Peru Methodology: A total of 131 stool samples were obtained from children under 5 years old hospitalized from January 2010 to December 2012 in the Hospital Regional de Cajamarca, Peru. ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were obtained by semi-nested multiplex RT-PCR and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 18.80% (22/117) tested positive for rotavirus by ELISA and 35.90% (42/117) by RT-PCR. Among the G-genotype identified, G9 in 35.71% (15/42) and G12 in 33.33% (14/42) were the most prevalent. With the most common combination being G12/P6 in 23.81% (10/42). Conclusions: A high prevalence of the G12/P6 genotype was detected. It is know that this genotype is not covered by the current vaccines available. More in depth studies are needed to know the current rotavirus genotypes presents in Peru.
Type:
info:eu-repo/semantics/bachelorThesis
Rights:
info:eu-repo/semantics/openAccess
Language:
spa
Keywords:
Rotavirus; Viral genotypes; Epidemiology; Acute gastroenteritis; Perú

Full metadata record

DC FieldValue Language
dc.contributor.advisorDel Valle Mendoza, Juana Mercedeses_PE
dc.contributor.authorWeilg Espejo, Pabloes_PE
dc.date.accessioned2017-02-06T14:49:41Z-
dc.date.available2017-02-06T14:49:41Z-
dc.date.issued30/01/2014-
dc.identifier.urihttp://hdl.handle.net/10757/620965-
dc.description.abstractBackground: Gastroenteritis by rotavirus is responsible for approximately 810 annual deaths/year in children under 5 years in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular-epidemiology of rotavirus diarrhea in Peru Methodology: A total of 131 stool samples were obtained from children under 5 years old hospitalized from January 2010 to December 2012 in the Hospital Regional de Cajamarca, Peru. ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were obtained by semi-nested multiplex RT-PCR and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 18.80% (22/117) tested positive for rotavirus by ELISA and 35.90% (42/117) by RT-PCR. Among the G-genotype identified, G9 in 35.71% (15/42) and G12 in 33.33% (14/42) were the most prevalent. With the most common combination being G12/P6 in 23.81% (10/42). Conclusions: A high prevalence of the G12/P6 genotype was detected. It is know that this genotype is not covered by the current vaccines available. More in depth studies are needed to know the current rotavirus genotypes presents in Peru.es_PE
dc.description.uriTesises_PE
dc.formatapplication/pdfes_PE
dc.formatapplication/mswordes_PE
dc.language.isospaes_PE
dc.publisherUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.relation.urlhttp://hdl.handle.net/10757/313901es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico UPCes_PE
dc.subjectRotaviruses_PE
dc.subjectViral genotypeses_PE
dc.subjectEpidemiologyes_PE
dc.subjectAcute gastroenteritises_PE
dc.subjectPerúes_PE
dc.titleDetección y genotipificación de rotavirus en pacientes con gastroenteritis agudaes_PE
dc.typeinfo:eu-repo/semantics/bachelorThesises_PE
thesis.degree.grantorUniversidad Peruana de Ciencias Aplicadas (UPC). Facultad de Ciencias de la Saludes_PE
thesis.degree.levelLicenciaturaes_PE
thesis.degree.disciplineMedicinaes_PE
thesis.degree.nameMédico cirujanoes_PE
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