Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis

2.50
Hdl Handle:
http://hdl.handle.net/10757/620656
Title:
Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis
Authors:
Camere Colarossi, Rosella; Ulloa Urizar, Gabriela; Medina Flores, Dyanne; Caballero García, Stefany; Mayta Tovalino, Frank; Del Valle Mendoza, Juana Mercedes ( 0000-0002-6011-5040 )
Citation:
Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis 2016, 6 (9):740 Asian Pacific Journal of Tropical Biomedicine
Publisher:
Elsevier B.V.
Journal:
Asian Pacific Journal of Tropical Biomedicine
Issue Date:
Sep-2016
URI:
http://hdl.handle.net/10757/620656
DOI:
10.1016/j.apjtb.2016.07.008
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S2221169116302635
Abstract:
Objective: To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia (Camu camu) (M. dubia) methanol extract, against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods: Two methanol extracts of M. dubia were prepared in vitro, from the seeds and pulp. Ten independent tests were prepared for each type of extract, using 0.12% chlorhexidine solution as positive control. Agar diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at 37 °C. Meanwhile, the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was found. Results: A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of (21.36 ± 6.35) mm and (19.21 ± 5.18) mm against S. mutans and S. sanguinis, respectively. The methanol extract of the pulp had an effect of (16.20 ± 2.08) mm and (19.34 ± 2.90) mm, respectively. The minimum inhibitory concentration of the pulp extract was 62.5 µg/mL for both strains, whereas for the seed antibacterial activity was observed even at low concentrations. The CC50 of the seeds extract was at a higher concentration than 800 µg/mL and 524.37 µg/mL for the pulp extract.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Antibacterial effect; Medicinal plants; Myrciaria dubia; Cytotoxity
ISSN:
22211691
Sponsors:
This study was supported by Universidad Peruana de Ciencias Aplicadas (UPC) Lima-Peru with Grant No. MANUSCRIPT ACCEPTED ACCEPTED MANUSCRIPT UPC-501-2015
Email:
jdelvall@upc.edu.pe

Full metadata record

DC FieldValue Language
dc.contributor.authorCamere Colarossi, Rosellaes_PE
dc.contributor.authorUlloa Urizar, Gabrielaes_PE
dc.contributor.authorMedina Flores, Dyannees_PE
dc.contributor.authorCaballero García, Stefanyes_PE
dc.contributor.authorMayta Tovalino, Frankes_PE
dc.contributor.authorDel Valle Mendoza, Juana Mercedeses_PE
dc.date.accessioned2016-10-05T14:27:50Z-
dc.date.available2016-10-05T14:27:50Z-
dc.date.issued2016-09-
dc.identifier.citationAntibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis 2016, 6 (9):740 Asian Pacific Journal of Tropical Biomedicinees_PE
dc.identifier.issn22211691-
dc.identifier.doi10.1016/j.apjtb.2016.07.008-
dc.identifier.urihttp://hdl.handle.net/10757/620656es_PE
dc.description.abstractObjective: To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia (Camu camu) (M. dubia) methanol extract, against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods: Two methanol extracts of M. dubia were prepared in vitro, from the seeds and pulp. Ten independent tests were prepared for each type of extract, using 0.12% chlorhexidine solution as positive control. Agar diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at 37 °C. Meanwhile, the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was found. Results: A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of (21.36 ± 6.35) mm and (19.21 ± 5.18) mm against S. mutans and S. sanguinis, respectively. The methanol extract of the pulp had an effect of (16.20 ± 2.08) mm and (19.34 ± 2.90) mm, respectively. The minimum inhibitory concentration of the pulp extract was 62.5 µg/mL for both strains, whereas for the seed antibacterial activity was observed even at low concentrations. The CC50 of the seeds extract was at a higher concentration than 800 µg/mL and 524.37 µg/mL for the pulp extract.es_PE
dc.description.sponsorshipThis study was supported by Universidad Peruana de Ciencias Aplicadas (UPC) Lima-Peru with Grant No. MANUSCRIPT ACCEPTED ACCEPTED MANUSCRIPT UPC-501-2015es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherElsevier B.V.es_PE
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2221169116302635es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectAntibacterial effectes_PE
dc.subjectMedicinal plantses_PE
dc.subjectMyrciaria dubiaes_PE
dc.subjectCytotoxityes_PE
dc.titleAntibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinises_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalAsian Pacific Journal of Tropical Biomedicinees_PE
dc.contributor.emailjdelvall@upc.edu.pees_PE
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