Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples

2.50
Hdl Handle:
http://hdl.handle.net/10757/605279
Title:
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples
Authors:
Gomes, Cláudia; Martinez Puchol, Sandra; Pons, Maria J.; Bazán, Jorge; Tinco, Carmen; Del Valle Mendoza, Juana Mercedes ( 0000-0002-6011-5040 ) ; Ruiz, Joaquim
Citation:
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples 2016, 10 (3):e0004529 PLOS Neglected Tropical Diseases
Journal:
PLOS Neglected Tropical Diseases
Issue Date:
9-Mar-2016
URI:
http://hdl.handle.net/10757/605279
DOI:
10.1371/journal.pntd.0004529
Additional Links:
http://dx.plos.org/10.1371/journal.pntd.0004529
Abstract:
Background The lack of an effective diagnostic tool for Carrion’s disease leads to misdiagnosis, wrong treatments and perpetuation of asymptomatic carriers living in endemic areas. Conventional PCR approaches have been reported as a diagnostic technique. However, the detection limit of these techniques is not clear as well as if its usefulness in low bacteriemia cases. The aim of this study was to evaluate the detection limit of 3 PCR approaches. Methodology/Principal Findings We determined the detection limit of 3 different PCR approaches: Bartonella-specific 16S rRNA, fla and its genes. We also evaluated the viability of dry blood spots to be used as a sample transport system. Our results show that 16S rRNA PCR is the approach with a lowest detection limit, 5 CFU/μL, and thus, the best diagnostic PCR tool studied. Dry blood spots diminish the sensitivity of the assay. Methodology/Principal Findings We determined the detection limit of 3 different PCR approaches: Bartonella-specific 16S rRNA, fla and its genes. We also evaluated the viability of dry blood spots to be used as a sample transport system. Our results show that 16S rRNA PCR is the approach with a lowest detection limit, 5 CFU/μL, and thus, the best diagnostic PCR tool studied. Dry blood spots diminish the sensitivity of the assay. Conclusions/Significance From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion’s disease. However its use in samples from dry blood spots results in easier management of transport samples in rural areas, a slight decrease in the sensitivity was observed. The usefulness to detect by PCR the presence of low-bacteriemic or asymptomatic carriers is doubtful, showing the need to search for new more sensible techniques.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Bastonella; Polymerase chain reaction; Carrion's disease; Ribosomal RNA; Blood; Diagnostic medicine; Gene amplification; Filter paper
ISSN:
1935-2735

Full metadata record

DC FieldValue Language
dc.contributor.authorGomes, Cláudiaes_PE
dc.contributor.authorMartinez Puchol, Sandraes_PE
dc.contributor.authorPons, Maria J.es_PE
dc.contributor.authorBazán, Jorgees_PE
dc.contributor.authorTinco, Carmenes_PE
dc.contributor.authorDel Valle Mendoza, Juana Mercedeses_PE
dc.contributor.authorRuiz, Joaquimes_PE
dc.date.accessioned2016-04-14T14:34:09Zes_PE
dc.date.available2016-04-14T14:34:09Zes_PE
dc.date.issued2016-03-09es_PE
dc.identifier.citationEvaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples 2016, 10 (3):e0004529 PLOS Neglected Tropical Diseaseses_PE
dc.identifier.issn1935-2735es_PE
dc.identifier.doi10.1371/journal.pntd.0004529es_PE
dc.identifier.urihttp://hdl.handle.net/10757/605279es_PE
dc.description.abstractBackground The lack of an effective diagnostic tool for Carrion’s disease leads to misdiagnosis, wrong treatments and perpetuation of asymptomatic carriers living in endemic areas. Conventional PCR approaches have been reported as a diagnostic technique. However, the detection limit of these techniques is not clear as well as if its usefulness in low bacteriemia cases. The aim of this study was to evaluate the detection limit of 3 PCR approaches. Methodology/Principal Findings We determined the detection limit of 3 different PCR approaches: Bartonella-specific 16S rRNA, fla and its genes. We also evaluated the viability of dry blood spots to be used as a sample transport system. Our results show that 16S rRNA PCR is the approach with a lowest detection limit, 5 CFU/μL, and thus, the best diagnostic PCR tool studied. Dry blood spots diminish the sensitivity of the assay. Methodology/Principal Findings We determined the detection limit of 3 different PCR approaches: Bartonella-specific 16S rRNA, fla and its genes. We also evaluated the viability of dry blood spots to be used as a sample transport system. Our results show that 16S rRNA PCR is the approach with a lowest detection limit, 5 CFU/μL, and thus, the best diagnostic PCR tool studied. Dry blood spots diminish the sensitivity of the assay. Conclusions/Significance From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion’s disease. However its use in samples from dry blood spots results in easier management of transport samples in rural areas, a slight decrease in the sensitivity was observed. The usefulness to detect by PCR the presence of low-bacteriemic or asymptomatic carriers is doubtful, showing the need to search for new more sensible techniques.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pntd.0004529es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectBastonellaes_PE
dc.subjectPolymerase chain reactiones_PE
dc.subjectCarrion's diseasees_PE
dc.subjectRibosomal RNAes_PE
dc.subjectBloodes_PE
dc.subjectDiagnostic medicinees_PE
dc.subjectGene amplificationes_PE
dc.subjectFilter paperes_PE
dc.titleEvaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Sampleses_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalPLOS Neglected Tropical Diseaseses_PE
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