Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis

4.00
Hdl Handle:
http://hdl.handle.net/10757/604437
Title:
Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis
Authors:
Medina Flores, Dyanne Adenea; Ulloa Urizar, Gabriela; Camere Colarossi, Rosella; Caballero García, Stefany; Mayta Tovalino, Frank; Del Valle Mendoza, Juana
Citation:
Medina-Flores D, Ulloa-Urizar G, Camere-Colarossi R, Caballero-García S, Mayta-Tovalino F, del Valle-Mendoza J, Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis, Asian Pacific Journal of Tropical Biomedicine (2016), doi: 10.1016/j.apjtb.2016.03.005.
Journal:
Asian Pacific Journal of Tropical Biomedicine (Asian Pac J Trop Biomed)
Issue Date:
Mar-2016
URI:
http://hdl.handle.net/10757/604437
DOI:
10.1016/j.apjtb.2016.03.005
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S2221169115309485
Abstract:
Objective To evaluate the cytotoxic and antibacterial effect of Bixa orellana L. (B. orellana) (achiote) methanol extract against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods Two methanol extracts of B. orellana were prepared in vitro, from the seeds and leaves. The antibacterial activity of extracts against S. mutans and S. sanguinis was evaluated using the cup-plate agar diffusion method. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxic activity was determinated by using the cell line MDCK. Results A stronger antibacterial effect was observed with the leaves methanolic extract with an inhibition zone of (19.97 ± 1.31) mm against S. mutans and (19.97 ± 1.26) mm against S. sanguinis. The methanolic extract of the seeds had an activity of (15.11 ± 1.03) mm and (16.15 ± 2.15) mm against S. mutans and S. sanguinis, respectively. The MIC of the leaf and the seed extracts against S. sanguinis was 62.5 and 125 μg/mL, respectively, and the MIC of the leaf extract against S. mutans was 62.5 μg/mL, and for the seed extract it was 31.25 μg/mL. The 50% cytotoxic concentration was 366.45 and 325.05 μg/mL for the leaves and seeds extracts, respectively. Conclusions The experimental findings demonstrated the antibacterial effect of the methanolic extract of B. orellana (achiote) on S. mutans and S. sanguinis. The extract of this plant is cytotoxic at high concentrations.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Antibacterial effect; Medicinal plants; Bixa orellana L; Cytotoxity
ISSN:
22211691; 2221-1691
Email:
jdelvall@upc.edu.pe

Full metadata record

DC FieldValue Language
dc.contributor.authorMedina Flores, Dyanne Adeneaes_PE
dc.contributor.authorUlloa Urizar, Gabrielaes_PE
dc.contributor.authorCamere Colarossi, Rosellaes_PE
dc.contributor.authorCaballero García, Stefanyes_PE
dc.contributor.authorMayta Tovalino, Frankes_PE
dc.contributor.authorDel Valle Mendoza, Juanaes_PE
dc.date.accessioned2016-04-04T20:37:04Zes_PE
dc.date.available2016-04-04T20:37:04Zes_PE
dc.date.issued2016-03es_PE
dc.identifier.citationMedina-Flores D, Ulloa-Urizar G, Camere-Colarossi R, Caballero-García S, Mayta-Tovalino F, del Valle-Mendoza J, Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis, Asian Pacific Journal of Tropical Biomedicine (2016), doi: 10.1016/j.apjtb.2016.03.005.es_PE
dc.identifier.issn22211691es_PE
dc.identifier.issn2221-1691es_PE
dc.identifier.doi10.1016/j.apjtb.2016.03.005es_PE
dc.identifier.urihttp://hdl.handle.net/10757/604437es_PE
dc.description.abstractObjective To evaluate the cytotoxic and antibacterial effect of Bixa orellana L. (B. orellana) (achiote) methanol extract against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods Two methanol extracts of B. orellana were prepared in vitro, from the seeds and leaves. The antibacterial activity of extracts against S. mutans and S. sanguinis was evaluated using the cup-plate agar diffusion method. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxic activity was determinated by using the cell line MDCK. Results A stronger antibacterial effect was observed with the leaves methanolic extract with an inhibition zone of (19.97 ± 1.31) mm against S. mutans and (19.97 ± 1.26) mm against S. sanguinis. The methanolic extract of the seeds had an activity of (15.11 ± 1.03) mm and (16.15 ± 2.15) mm against S. mutans and S. sanguinis, respectively. The MIC of the leaf and the seed extracts against S. sanguinis was 62.5 and 125 μg/mL, respectively, and the MIC of the leaf extract against S. mutans was 62.5 μg/mL, and for the seed extract it was 31.25 μg/mL. The 50% cytotoxic concentration was 366.45 and 325.05 μg/mL for the leaves and seeds extracts, respectively. Conclusions The experimental findings demonstrated the antibacterial effect of the methanolic extract of B. orellana (achiote) on S. mutans and S. sanguinis. The extract of this plant is cytotoxic at high concentrations.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2221169115309485es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectAntibacterial effectes_PE
dc.subjectMedicinal plantses_PE
dc.subjectBixa orellana Les_PE
dc.subjectCytotoxityes_PE
dc.titleAntibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinises_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalAsian Pacific Journal of Tropical Biomedicine (Asian Pac J Trop Biomed)es_PE
dc.description.peer-reviewPeer reviewes_PE
dc.contributor.emailjdelvall@upc.edu.pees_PE
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