Multi-Locus Sequence Typing of Bartonella bacilliformis DNA Performed Directly from Blood of Patients with Oroya's Fever During a Peruvian Outbreak.

2.50
Hdl Handle:
http://hdl.handle.net/10757/595423
Title:
Multi-Locus Sequence Typing of Bartonella bacilliformis DNA Performed Directly from Blood of Patients with Oroya's Fever During a Peruvian Outbreak.
Authors:
Pons, Maria J; Silva-Caso, Wilmer; Del Valle Mendoza, Juana; Ruiz, Joaquim
Citation:
Multi-Locus Sequence Typing of Bartonella bacilliformis DNA Performed Directly from Blood of Patients with Oroya's Fever During a Peruvian Outbreak. 2016, 10 (1):e0004391 PLoS Negl Trop Dis
Publisher:
PLoS ONE
Journal:
PLoS neglected tropical diseases
Issue Date:
1-Feb-2016
URI:
http://hdl.handle.net/10757/595423
DOI:
10.1371/journal.pntd.0004391
PubMed ID:
26824740
Additional Links:
http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004391
Abstract:
Background Bartonella bacilliformis is the etiological agent of Carrion’s disease, a neglected tropical poverty-linked illness. This infection is endemic of Andean regions and it is estimated that approximately 1.7 million of South Americans are at risk. This bacterium is a fastidious slow growing microorganism, which is difficult and cumbersome to isolate from clinical sources, thereby hindering the availability of phylogenetic relationship of clinical samples. The aim of this study was to perform Multi Locus Sequence Typing of B. bacilliformis directly in blood from patients diagnosed with Oroya fever during an outbreak in Northern Peru. Methodology/Principal Findings DNA extracted among blood samples from patients diagnosed with Oroya’s fever were analyzed with MLST, with the amplification of 7 genetic loci (ftsZ, flaA, ribC, rnpB, rpoB, bvrR and groEL) and a phylogenetic analysis of the different Sequence Types (ST) was performed. A total of 4 different ST were identified. The most frequently found was ST1 present in 66% of samples. Additionally, two samples presented a new allelic profile, belonging to new STs (ST 9 and ST 10), which were closely related to ST1. Conclusions/Significance The present data demonstrate that B. bacilliformis MLST studies may be possible directly from blood samples, being a promising approach for epidemiological studies. During the outbreak the STs of B. bacilliformis were found to be heterogeneous, albeit closely related, probably reflecting the evolution from a common ancestor colonizing the area. Additional studies including new samples and areas are needed, in order to obtain better knowledge of phylogenetic scenario B. bacilliformis
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Bastonella; Fever; Blood; Peru; Phylogenetics
ISSN:
1935-2735

Full metadata record

DC FieldValue Language
dc.contributor.authorPons, Maria Jes_PE
dc.contributor.authorSilva-Caso, Wilmeres_PE
dc.contributor.authorDel Valle Mendoza, Juanaes_PE
dc.contributor.authorRuiz, Joaquimes_PE
dc.date.accessioned2016-02-01T19:44:58Zes_PE
dc.date.available2016-02-01T19:44:58Zes_PE
dc.date.issued2016-02-01es_PE
dc.identifier.citationMulti-Locus Sequence Typing of Bartonella bacilliformis DNA Performed Directly from Blood of Patients with Oroya's Fever During a Peruvian Outbreak. 2016, 10 (1):e0004391 PLoS Negl Trop Dises_PE
dc.identifier.issn1935-2735es_PE
dc.identifier.pmid26824740es_PE
dc.identifier.doi10.1371/journal.pntd.0004391es_PE
dc.identifier.urihttp://hdl.handle.net/10757/595423es_PE
dc.description.abstractBackground Bartonella bacilliformis is the etiological agent of Carrion’s disease, a neglected tropical poverty-linked illness. This infection is endemic of Andean regions and it is estimated that approximately 1.7 million of South Americans are at risk. This bacterium is a fastidious slow growing microorganism, which is difficult and cumbersome to isolate from clinical sources, thereby hindering the availability of phylogenetic relationship of clinical samples. The aim of this study was to perform Multi Locus Sequence Typing of B. bacilliformis directly in blood from patients diagnosed with Oroya fever during an outbreak in Northern Peru. Methodology/Principal Findings DNA extracted among blood samples from patients diagnosed with Oroya’s fever were analyzed with MLST, with the amplification of 7 genetic loci (ftsZ, flaA, ribC, rnpB, rpoB, bvrR and groEL) and a phylogenetic analysis of the different Sequence Types (ST) was performed. A total of 4 different ST were identified. The most frequently found was ST1 present in 66% of samples. Additionally, two samples presented a new allelic profile, belonging to new STs (ST 9 and ST 10), which were closely related to ST1. Conclusions/Significance The present data demonstrate that B. bacilliformis MLST studies may be possible directly from blood samples, being a promising approach for epidemiological studies. During the outbreak the STs of B. bacilliformis were found to be heterogeneous, albeit closely related, probably reflecting the evolution from a common ancestor colonizing the area. Additional studies including new samples and areas are needed, in order to obtain better knowledge of phylogenetic scenario B. bacilliformises_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherPLoS ONEes_PE
dc.relation.urlhttp://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004391es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectBastonellaes_PE
dc.subjectFeveres_PE
dc.subjectBloodes_PE
dc.subjectPerues_PE
dc.subjectPhylogeneticses_PE
dc.titleMulti-Locus Sequence Typing of Bartonella bacilliformis DNA Performed Directly from Blood of Patients with Oroya's Fever During a Peruvian Outbreak.es_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalPLoS neglected tropical diseaseses_PE
dc.description.fundingThis work has been supported by the Spanish Network for the Research in Infectious Diseases [REIPI RD12/0015],by Generalitat de Catalunya, Departament d’Universitats, Recerca i Societat de la Informació [2014 SGR 26] and by by a grant of the Instituto de Salud Carlos III - Spain (PI11/ 00983) which included FEDER funds (JR). MJP has a postdoctoral fellowship from CONCYTEC/ FONDECYT (grant number: CG05-2013- FONDECYT). JR has a fellowship from the programes_PE
dc.description.peer-reviewRevisión por pareses_PE
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