Evaluación in vitro del efecto antimicrobiano de los probióticos lactobacillus rhamnosus GG (ATCC 53103) y bifidobacterium bifidum (ATCC 11863) contra streptococcus mutans (ATCC 25175) y streptococcus sanguinis (ATCC 10556)

2.50
Hdl Handle:
http://hdl.handle.net/10757/582435
Title:
Evaluación in vitro del efecto antimicrobiano de los probióticos lactobacillus rhamnosus GG (ATCC 53103) y bifidobacterium bifidum (ATCC 11863) contra streptococcus mutans (ATCC 25175) y streptococcus sanguinis (ATCC 10556)
Authors:
De Lama Odría, María del Carmen
Advisors:
Del Valle Mendoza, Juana Mercedes
Citation:
De Lama Odría M del C. Evaluación in vitro del efecto antimicrobiano de los probióticos lactobacillus rhamnosus GG (ATCC 53103) y bifidobacterium bifidum (ATCC 11863) contra streptococcus mutans (ATCC 25175) y streptococcus sanguinis (ATCC 10556) [Internet]. Universidad Peruana de Ciencias Aplicadas - UPC; 2015 [cited 2016 May 5]. Available from: http://repositorioacademico.upc.edu.pe/upc/handle/10757/582435
Publisher:
Universidad Peruana de Ciencias Aplicadas (UPC)
Issue Date:
19
URI:
http://hdl.handle.net/10757/582435
Abstract:
Introduction: Nowadays there is a high consumption of probiotics in food products due to its ability to fight pathogens. However, less is known of the effects of probiotics in oral microbial ecology. Aim: To determinate the antimicrobial effect of the probiotics Lactobacillus rhamnosus GG and Bifidobacterium bifidum against the cariogenic bacteria Streptococcus mutans and its antagonist Streptoccoccus sanguinis. Material and methods: For the present experimental study in vitro, strains of S. mutans (ATCC 27175), S. sanguinis (ATCC10556), LGG (ATCC 53103) and Bifidobacterium bifidum (ATCC 11863) were used. The two first bacteria were grown in Brain Heart Infusion broth (BHI) and the rest in Man Rogosa Sharpe (MRS). The samples were incubated under anaerobic conditions at 37 °C for 72 hours. For inhibition tests, four groups were prepared: S. mutans inhibition by LGG and B. bifidum (1); S. sanguinis inhibition by LGG and B. bifidum (2); LGG inhibition by S. mutans and S. sanguinis (3) and B. bifidum inhibition by S. mutans and S. sanguinis (4). The inhibitory effect was evaluated with the cup-plate diffusion method in which each well contained 100 µL of the bacteria selected as inhibitory agent. The wells were made on a BHI agar containing the microorganism to be inhibited. Chlorhexidine was the positive control of agar bacterial growth and periopathogenic bacteria the indicators of the antibacterial effect of probiotics.
Abstract:
Introducción: actualmente, existe un alto consumo de probióticos debido a su capacidad de combatir microorganismos patógenos. Pese a ello, existe poca evidencia científica del efecto de éstos sobre la microbiota oral. Objetivo: evaluar la capacidad antimicrobiana del Lactobacillus rhamnosus GG (LGG) y del Bifidobacterium bifidum frente a la bacteria cariogénica Streptococcus mutans y su antagonista Streptoccoccus sanguinis. Materiales y Métodos: para este estudio experimental in vitro, se utilizaron cepas de S. mutans (ATCC 27175), S. sanguinis (ATCC10556), LGG (ATCC 53103) y Bifidobacterium bifidum (ATCC 11863). Las dos primeras cultivadas en caldo Brain Heart Infusion (BHI) y las dos últimas en Man Rogosa Sharpe (MRS), incubadas en condiciones de anaerobiosis, a 37ºC, durante 72 horas. Se prepararon 4 grupos de estudio: prueba de inhibición de S. mutans por LGG y B. bifidum (grupo 1); de S. sanguinis por LGG y B. bifidum (grupo 2); de LGG por S. mutans y S. sanguinis (grupo 3) y de B. bifidum por S. mutans y S. sanguinis (grupo 4). El efecto inhibitorio se evaluó con la prueba de difusión con pocillos, los cuales contenían el inóculo de la bacteria seleccionada como agente antibacteriano. El agar BHI contenía el cultivo del microorganismo que se buscaba inhibir. La clorhexidina fue el control positivo del crecimiento bacteriano en agar y las bacterias periopatógenas los cultivos indicadores del efecto antibacteriano de los probióticos. Resultados: el LGG y el B. bifidum demostraron no tener un efecto antibacteriano frente al S. mutans y S. sanguinis. Conclusiones: los probióticos LGG y Bifidobacterium bifidum no poseen un efecto antibacteriano contra Streptococcus mutans y Streptococcus sanguinis, siendo de relevancia clínica para la adecuada elección de microorganismos para las nuevas estrategias preventivas de caries en base a terapias de reemplazo.
Type:
info:eu-repo/semantics/bachelorThesis
Rights:
info:eu-repo/semantics/embargoedAccess
Language:
spa
Keywords:
Probióticos; Antibacterianos; Streptococcus Mutants; Streptococcus Sanguis; Odontología; Tesis

Full metadata record

DC FieldValue Language
dc.contributor.advisorDel Valle Mendoza, Juana Mercedeses_PE
dc.contributor.authorDe Lama Odría, María del Carmenes_PE
dc.date.accessioned2015-11-20T02:36:06Zes_PE
dc.date.available2015-11-20T02:36:06Zes_PE
dc.date.issued19/11/2015es_PE
dc.identifier.citationDe Lama Odría M del C. Evaluación in vitro del efecto antimicrobiano de los probióticos lactobacillus rhamnosus GG (ATCC 53103) y bifidobacterium bifidum (ATCC 11863) contra streptococcus mutans (ATCC 25175) y streptococcus sanguinis (ATCC 10556) [Internet]. Universidad Peruana de Ciencias Aplicadas - UPC; 2015 [cited 2016 May 5]. Available from: http://repositorioacademico.upc.edu.pe/upc/handle/10757/582435es_PE
dc.identifier.urihttp://hdl.handle.net/10757/582435es_PE
dc.description.abstractIntroduction: Nowadays there is a high consumption of probiotics in food products due to its ability to fight pathogens. However, less is known of the effects of probiotics in oral microbial ecology. Aim: To determinate the antimicrobial effect of the probiotics Lactobacillus rhamnosus GG and Bifidobacterium bifidum against the cariogenic bacteria Streptococcus mutans and its antagonist Streptoccoccus sanguinis. Material and methods: For the present experimental study in vitro, strains of S. mutans (ATCC 27175), S. sanguinis (ATCC10556), LGG (ATCC 53103) and Bifidobacterium bifidum (ATCC 11863) were used. The two first bacteria were grown in Brain Heart Infusion broth (BHI) and the rest in Man Rogosa Sharpe (MRS). The samples were incubated under anaerobic conditions at 37 °C for 72 hours. For inhibition tests, four groups were prepared: S. mutans inhibition by LGG and B. bifidum (1); S. sanguinis inhibition by LGG and B. bifidum (2); LGG inhibition by S. mutans and S. sanguinis (3) and B. bifidum inhibition by S. mutans and S. sanguinis (4). The inhibitory effect was evaluated with the cup-plate diffusion method in which each well contained 100 µL of the bacteria selected as inhibitory agent. The wells were made on a BHI agar containing the microorganism to be inhibited. Chlorhexidine was the positive control of agar bacterial growth and periopathogenic bacteria the indicators of the antibacterial effect of probiotics.en
dc.description.abstractIntroducción: actualmente, existe un alto consumo de probióticos debido a su capacidad de combatir microorganismos patógenos. Pese a ello, existe poca evidencia científica del efecto de éstos sobre la microbiota oral. Objetivo: evaluar la capacidad antimicrobiana del Lactobacillus rhamnosus GG (LGG) y del Bifidobacterium bifidum frente a la bacteria cariogénica Streptococcus mutans y su antagonista Streptoccoccus sanguinis. Materiales y Métodos: para este estudio experimental in vitro, se utilizaron cepas de S. mutans (ATCC 27175), S. sanguinis (ATCC10556), LGG (ATCC 53103) y Bifidobacterium bifidum (ATCC 11863). Las dos primeras cultivadas en caldo Brain Heart Infusion (BHI) y las dos últimas en Man Rogosa Sharpe (MRS), incubadas en condiciones de anaerobiosis, a 37ºC, durante 72 horas. Se prepararon 4 grupos de estudio: prueba de inhibición de S. mutans por LGG y B. bifidum (grupo 1); de S. sanguinis por LGG y B. bifidum (grupo 2); de LGG por S. mutans y S. sanguinis (grupo 3) y de B. bifidum por S. mutans y S. sanguinis (grupo 4). El efecto inhibitorio se evaluó con la prueba de difusión con pocillos, los cuales contenían el inóculo de la bacteria seleccionada como agente antibacteriano. El agar BHI contenía el cultivo del microorganismo que se buscaba inhibir. La clorhexidina fue el control positivo del crecimiento bacteriano en agar y las bacterias periopatógenas los cultivos indicadores del efecto antibacteriano de los probióticos. Resultados: el LGG y el B. bifidum demostraron no tener un efecto antibacteriano frente al S. mutans y S. sanguinis. Conclusiones: los probióticos LGG y Bifidobacterium bifidum no poseen un efecto antibacteriano contra Streptococcus mutans y Streptococcus sanguinis, siendo de relevancia clínica para la adecuada elección de microorganismos para las nuevas estrategias preventivas de caries en base a terapias de reemplazo.es_PE
dc.description.uriTesises_PE
dc.formatapplication/pdfes_PE
dc.formatapplication/mswordes_PE
dc.language.isospaes_PE
dc.publisherUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico UPCes_PE
dc.subjectProbióticoses_PE
dc.subjectAntibacterianoses_PE
dc.subjectStreptococcus Mutantses_PE
dc.subjectStreptococcus Sanguises_PE
dc.subjectOdontologíaes_PE
dc.subjectTesises_PE
dc.titleEvaluación in vitro del efecto antimicrobiano de los probióticos lactobacillus rhamnosus GG (ATCC 53103) y bifidobacterium bifidum (ATCC 11863) contra streptococcus mutans (ATCC 25175) y streptococcus sanguinis (ATCC 10556)es_PE
dc.typeinfo:eu-repo/semantics/bachelorThesises_PE
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A randomized clinical trial of the effect of yoghurt on the human salivary microbiota. Arch Oral Biol. 2001; 46: 705-12. 52. Hojo K. Nagaoka S. Murata S. Takemoto N, Ohshima T, Maeda N. Reduction of vitamin K concentration by salivary Bifidobacterium strains and their possible nutritional competition with Porphyromonas gingivalis. J Appl Microbiol. 2007; 103(5): 1969-74.es_PE
thesis.degree.grantorUniversidad Peruana de Ciencias Aplicadas (UPC). Facultad de Ciencias de la Saludes_PE
thesis.degree.levelLicenciaturaes_PE
thesis.degree.disciplineOdontologíaes_PE
thesis.degree.nameCirujano dentistaes_PE
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