Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak

2.50
Hdl Handle:
http://hdl.handle.net/10757/582375
Title:
Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
Authors:
Pons, Maria J.; Silva, Wilmer; Gomes, Cláudia; Ruiz, Joaquim; Del Valle Mendoza, Juana Mercedes ( 0000-0002-6011-5040 )
Publisher:
Universidad Peruana de Ciencias Aplicadas (UPC)
Issue Date:
18-Nov-2015
URI:
http://hdl.handle.net/10757/582375
Abstract:
The bacteria Bartonella bacilliformis is the etiological agent of Carrion’s disease, which is a neglected poverty-related disease, affecting Mountain Andean valleys of Peru, Colombia and Ecuador. This disease, in absence of treatment presents a high mortality during the acute phase, called Oroya’s Fever. The second phase is characterized by the development of dermal eruptions, known as “Peruvian wart”. This bacterium is a fastidious slow growing microorganism, being difficult and cumbersome to culture and isolate from clinical sources. Then, the available data about phylogenetic relationship in clinical samples are really scarce, but suggesting high variability. The aim of the study was to perform direct blood analysis of B. bacilliformis Multi Locus Sequence Typing (MLST), a genotyping tool, in patients with Oroya fever during an outbreak. The present study demonstrate that the direct blood MLST PCR is a technique useful in the phylogenic characterization of this fastidious microorganism endemic from Andean regions. In this study, we demonstrate that the outbreak of Oroya’s fever was caused by closely related Sequence Typing (ST) microorganisms and, additionally, new STs have been described.
Type:
info:eu-repo/semantics/conferenceObject
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Description:
ASTMH 64th Annual Meeting. October 25-29, 2015 Philadelphia Marriott Downtown Philadelphia, Pennsylvania USA
Keywords:
Bartonella bacilliformis; Peru

Full metadata record

DC FieldValue Language
dc.contributor.authorPons, Maria J.es_PE
dc.contributor.authorSilva, Wilmeres_PE
dc.contributor.authorGomes, Cláudiaes_PE
dc.contributor.authorRuiz, Joaquimes_PE
dc.contributor.authorDel Valle Mendoza, Juana Mercedeses_PE
dc.creatorUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.date.accessioned2015-11-18T21:10:38Zes_PE
dc.date.available2015-11-18T21:10:38Zes_PE
dc.date.issued2015-11-18es_PE
dc.identifier.urihttp://hdl.handle.net/10757/582375es_PE
dc.descriptionASTMH 64th Annual Meeting. October 25-29, 2015 Philadelphia Marriott Downtown Philadelphia, Pennsylvania USAeng
dc.description.abstractThe bacteria Bartonella bacilliformis is the etiological agent of Carrion’s disease, which is a neglected poverty-related disease, affecting Mountain Andean valleys of Peru, Colombia and Ecuador. This disease, in absence of treatment presents a high mortality during the acute phase, called Oroya’s Fever. The second phase is characterized by the development of dermal eruptions, known as “Peruvian wart”. This bacterium is a fastidious slow growing microorganism, being difficult and cumbersome to culture and isolate from clinical sources. Then, the available data about phylogenetic relationship in clinical samples are really scarce, but suggesting high variability. The aim of the study was to perform direct blood analysis of B. bacilliformis Multi Locus Sequence Typing (MLST), a genotyping tool, in patients with Oroya fever during an outbreak. The present study demonstrate that the direct blood MLST PCR is a technique useful in the phylogenic characterization of this fastidious microorganism endemic from Andean regions. In this study, we demonstrate that the outbreak of Oroya’s fever was caused by closely related Sequence Typing (ST) microorganisms and, additionally, new STs have been described.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectBartonella bacilliformises_PE
dc.subjectPerues_PE
dc.titleDirect blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreakes_PE
dc.typeinfo:eu-repo/semantics/conferenceObjectes_PE
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