Development and analysis of furazolidone-resistant Escherichia coli mutants

2.50
Hdl Handle:
http://hdl.handle.net/10757/556955
Title:
Development and analysis of furazolidone-resistant Escherichia coli mutants
Authors:
Martínez Puchol, Sandra; Gómes, Cláudia; Pons, Maria J.; Ruíz Roldan, Lidia; Torrents De La Peña, Alba; Ochoa, Theresa J.; Ruíz, Joaquim
Citation:
Mart ınez-Puchol S, Gomes C, Pons MJ, Ruiz-Rold an L, Torrents de la Pe~na A, Ochoa TJ, Ruiz J. Development and analysis of furazolidone-resistant Escherichia coli mutants. APMIS 2015.
Publisher:
John Wiley & Sons, Inc.
Journal:
Acta pathologica, microbiologica et Immunológica Scandinavica (APMIS.)
Issue Date:
15-Jun-2015
URI:
http://hdl.handle.net/10757/556955
DOI:
10.1111/apm.12401
PubMed ID:
26011027
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/26011027
Abstract:
Furazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe-Arg-β-Naphtylamyde. The presence of mutations in the nitroreductase genes nfsA and nfsB was analysed by PCR; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by SDS-PAGE. The frequency of mutation ranged from <9.6 × 10-10 to 9.59 × 10-7 . Neither an effect on efflux pumps inhibited by Phe-Arg-β-Naphtylamyde nor cross-resistance with the antibiotics studied was observed. Nineteen mutants (52.94%) presented mutations in the nitroreductase-encoding genes: 17 in the nfsA gene (15 mutants with an internal stop codon, 2 with amino acid changes), 2 in the nfsB (all amino acid changes). Alterations in the outer membrane proteins OmpA and OmpW were also observed. Although more studies are necessary to find other resistance mechanisms, present data showed the low potential of selecting furazolidone-resistant mutants, together with the lack of cross-resistance with unrelated antimicrobial agents.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Description:
Revisión por pares; joruiz@clinic.ub.es
Keywords:
Enterobacteriaceae; Nitrofuran; Resistance mechanisms
ISSN:
1600-0463

Full metadata record

DC FieldValue Language
dc.contributor.authorMartínez Puchol, Sandraes_PE
dc.contributor.authorGómes, Cláudiaes_PE
dc.contributor.authorPons, Maria J.es_PE
dc.contributor.authorRuíz Roldan, Lidiaes_PE
dc.contributor.authorTorrents De La Peña, Albaes_PE
dc.contributor.authorOchoa, Theresa J.es_PE
dc.contributor.authorRuíz, Joaquimes_PE
dc.date.accessioned2015-06-15T16:20:48Zes_PE
dc.date.available2015-06-15T16:20:48Zes_PE
dc.date.issued2015-06-15es_PE
dc.identifier.citationMart ınez-Puchol S, Gomes C, Pons MJ, Ruiz-Rold an L, Torrents de la Pe~na A, Ochoa TJ, Ruiz J. Development and analysis of furazolidone-resistant Escherichia coli mutants. APMIS 2015.es_PE
dc.identifier.issn1600-0463es_PE
dc.identifier.pmid26011027es_PE
dc.identifier.doi10.1111/apm.12401es_PE
dc.identifier.urihttp://hdl.handle.net/10757/556955es_PE
dc.descriptionRevisión por pareses_PE
dc.descriptionjoruiz@clinic.ub.eses_PE
dc.description.abstractFurazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe-Arg-β-Naphtylamyde. The presence of mutations in the nitroreductase genes nfsA and nfsB was analysed by PCR; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by SDS-PAGE. The frequency of mutation ranged from <9.6 × 10-10 to 9.59 × 10-7 . Neither an effect on efflux pumps inhibited by Phe-Arg-β-Naphtylamyde nor cross-resistance with the antibiotics studied was observed. Nineteen mutants (52.94%) presented mutations in the nitroreductase-encoding genes: 17 in the nfsA gene (15 mutants with an internal stop codon, 2 with amino acid changes), 2 in the nfsB (all amino acid changes). Alterations in the outer membrane proteins OmpA and OmpW were also observed. Although more studies are necessary to find other resistance mechanisms, present data showed the low potential of selecting furazolidone-resistant mutants, together with the lack of cross-resistance with unrelated antimicrobial agents.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherJohn Wiley & Sons, Inc.es_PE
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/26011027es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectEnterobacteriaceaees_PE
dc.subjectNitrofuranes_PE
dc.subjectResistance mechanismses_PE
dc.titleDevelopment and analysis of furazolidone-resistant Escherichia coli mutantses_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalActa pathologica, microbiologica et Immunológica Scandinavica (APMIS.)es_PE
dc.description.fundingThis work was supported by the Agencia Espa~nola de Cooperaci on Internacional al Desarrollo (AECID) [grants nos. D/024648/09 and D/030509/10] and by Generalitat de Catalunya, Departament d’Universitats, Recerca i Societat de la Informaci o (2014 SGR 26). JR has a fellowship from the programme I3 of the Instituto de Salud Carlos III (ISCIII, Spain) [grant no. CES11/012].es_PE

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