The role of viruses in the aetiology of IRA in Peruvian children

2.50
Hdl Handle:
http://hdl.handle.net/10757/347234
Title:
The role of viruses in the aetiology of IRA in Peruvian children
Authors:
Del Valle Mendoza, Juana; Cornejo Tapia, Ángela; Del Valle, L.; Pumarola, T.; Verne, E.; Helasvuo, V.; Nazario, R.; Champin, Denisse
Citation:
1. Ideh RC, Rodrigues O, Armah GE, Batchilly AS. Paediatric rotavirus disease in The Gambia: A hospital based sentinel study. Int J Infect Dis [Internet]. 2010;14(Icid):e422–3. Available from: http://hdl.handle.net/10757/347234
Publisher:
Elsevier B.V.
Journal:
International Journal of Infectious Diseases
Issue Date:
27-Mar-2015
URI:
http://hdl.handle.net/10757/347234
Additional Links:
http://www.sciencedirect.com/science/article/pii/S120197121000593X?np=y
Type:
info:eu-repo/semantics/conferenceObject
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Description:
Background: The role of respiratory viruses in community may have been previously underestimated. We aimed to study the incidence and clinical characteristics of acute respiratory infections (IRA) in children adding PCR to routine conventional laboratory tests. Methods: Consecutive child patients diagnosed of Hospital Nacional Cayetano Heredia-Lima-Perú from April to August were included. Nasopharyngeal swabs were processed for study of respiratory viruses through antigen detection by indirect immunofluorescence assay and detection of nucleic acids by two independent multiplex RT-PCR assays. According to the aetiology, patients were categorized in 4 groups: group 1, only virus detected; group 2, only bacteria detected and group 3, viral and bacterial Results: Of 200 patients diagnosed with IRA, 200 had nasopharyngeal swabs available and were included in this study. Aetiology was established in 200 patients: group 1, n=57 (28.5%); group 2, n= 23 (11.5%); group 3, n= 25(12.5%). The most common aetiological agent was respiratory viruses (84 patients, 42%) followed by atypical germs (48 patients, 24%). Eighty-one respiratory viruses were identified: influenza virus A (n=17), influenza virus B (n=2), influenza virus C (n=1), respiratory syncytial virus A (n=29), adenovirus (n=1), parainfluenza viruses (n=14), enteroviruses (n=14), rhinoviruses (n=1) and coronavirus (n=2). There were eleven patients coinfected with respiratory virus. Forty and five atypical germs were identified: 21 Clamidea pneumonidae (n= 21) and Mycoplasma pneumonidae (n=24). There were sixteen patients coinfected by both atypical germs. Immunofluorescence 41 and PCR 81. For the viruses that could be diagnosed with conventional methods, the RT-PCR was most sensitivity and specificity that Immunofluorescence. Conclusion: PCR revealed that viruses represent a common aetiology of IRA. There is an urgent need to reconsider routine laboratory tests for an adequate diagnosis of respiratory viruses, as clinical characteristics are unable to reliably distinguish viral from bacterial aetiology.
Keywords:
Virus; Children
ISSN:
10.1016/j.ijid.2010.02.561

Full metadata record

DC FieldValue Language
dc.contributor.authorDel Valle Mendoza, Juanaes_PE
dc.contributor.authorCornejo Tapia, Ángelaes_PE
dc.contributor.authorDel Valle, L.es_PE
dc.contributor.authorPumarola, T.es_PE
dc.contributor.authorVerne, E.es_PE
dc.contributor.authorHelasvuo, V.es_PE
dc.contributor.authorNazario, R.es_PE
dc.contributor.authorChampin, Denissees_PE
dc.creatorUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.date.accessioned2015-03-27T22:36:24Zes_PE
dc.date.available2015-03-27T22:36:24Zes_PE
dc.date.issued2015-03-27es_PE
dc.identifier.citation1. Ideh RC, Rodrigues O, Armah GE, Batchilly AS. Paediatric rotavirus disease in The Gambia: A hospital based sentinel study. Int J Infect Dis [Internet]. 2010;14(Icid):e422–3. Available from: http://hdl.handle.net/10757/347234es_PE
dc.identifier.issn10.1016/j.ijid.2010.02.561es_PE
dc.identifier.urihttp://hdl.handle.net/10757/347234es_PE
dc.descriptionBackground: The role of respiratory viruses in community may have been previously underestimated. We aimed to study the incidence and clinical characteristics of acute respiratory infections (IRA) in children adding PCR to routine conventional laboratory tests. Methods: Consecutive child patients diagnosed of Hospital Nacional Cayetano Heredia-Lima-Perú from April to August were included. Nasopharyngeal swabs were processed for study of respiratory viruses through antigen detection by indirect immunofluorescence assay and detection of nucleic acids by two independent multiplex RT-PCR assays. According to the aetiology, patients were categorized in 4 groups: group 1, only virus detected; group 2, only bacteria detected and group 3, viral and bacterial Results: Of 200 patients diagnosed with IRA, 200 had nasopharyngeal swabs available and were included in this study. Aetiology was established in 200 patients: group 1, n=57 (28.5%); group 2, n= 23 (11.5%); group 3, n= 25(12.5%). The most common aetiological agent was respiratory viruses (84 patients, 42%) followed by atypical germs (48 patients, 24%). Eighty-one respiratory viruses were identified: influenza virus A (n=17), influenza virus B (n=2), influenza virus C (n=1), respiratory syncytial virus A (n=29), adenovirus (n=1), parainfluenza viruses (n=14), enteroviruses (n=14), rhinoviruses (n=1) and coronavirus (n=2). There were eleven patients coinfected with respiratory virus. Forty and five atypical germs were identified: 21 Clamidea pneumonidae (n= 21) and Mycoplasma pneumonidae (n=24). There were sixteen patients coinfected by both atypical germs. Immunofluorescence 41 and PCR 81. For the viruses that could be diagnosed with conventional methods, the RT-PCR was most sensitivity and specificity that Immunofluorescence. Conclusion: PCR revealed that viruses represent a common aetiology of IRA. There is an urgent need to reconsider routine laboratory tests for an adequate diagnosis of respiratory viruses, as clinical characteristics are unable to reliably distinguish viral from bacterial aetiology.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherElsevier B.V.es_PE
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S120197121000593X?np=yes_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectViruses_PE
dc.subjectChildrenes_PE
dc.titleThe role of viruses in the aetiology of IRA in Peruvian childrenes_PE
dc.typeinfo:eu-repo/semantics/conferenceObjectes_PE
dc.identifier.journalInternational Journal of Infectious Diseaseses_PE
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