Characterisation of extended-spectrum b-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique

2.50
Hdl Handle:
http://hdl.handle.net/10757/347017
Title:
Characterisation of extended-spectrum b-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique
Authors:
Pons, Maria J.; Vubil, Delfino; Guiral, Elisabet; Jaintilal, Dinis; Fraile, Oscar; Soto, Sara M.; Sigauque, Betuel; Nhampossa, Tacilta; Aide, Pedro; Alonso, Pedro L.; Vila, Jordi; Mandomando, Inacio; Ruiz, Joaquim
Citation:
Pons MJ, Vubil D, Guiral E, Jaintilal D, Fraile O, Soto SM, et al. Characterisation of extended-spectrum β-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique. J Glob Antimicrob Resist [Internet]. 2015;3:19–25. Available from: http://linkinghub.elsevier.com/retrieve/pii/S2213716515000065
Publisher:
Elsevier B.V.
Journal:
Journal of Global Antimicrobial Resistance
Issue Date:
23-Mar-2015
URI:
http://hdl.handle.net/10757/347017
DOI:
10.1016/j.jgar.2015.01.004
Additional Links:
http://www.sciencedirect.com/science/article/pii/S2213716515000065
Abstract:
The aim of this study was to determine the prevalence of extended-spectrum b-lactamase (ESBL)- producing Klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in Manhic¸a, Mozambique. ESBLs were investigated among ceftriaxone-non-susceptible K. pneumoniae clinical isolates recovered between 2004 and 2009. Characterisation of blaCTX-M, blaSHV, blaOXA and blaTEM genes was performed by PCR and sequencing. Epidemiological relationships were established by phylogenetic analysis, repetitive extragenic palindromic PCR (REP-PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), whilst plasmid transferability was evaluated by conjugation. In addition,the presence of class 1 and 2 integrons was studied.A total of 19 K. pneumoniae were analysed. The blaCTX-M-15 gene was found in all strains. Other ESBL genes were found concomitantly, including blaSHV-5, blaSHV-2, blaSHV-2A, blaSHV-12 and blaSHV-38. In addition, other b-lactamases such as blaTEM-1 and blaOXA-30 were also detected. REP-PCR identified 15 different epidemiological profiles. MLST analysis also showed great variability of sequence types. The blaCTX-M-15 gene showed a high transfer capacity. The presence of class 1 integrons was high. High levels of multidrug resistance were also found. In conclusion, these data show the dominance of the CTX-M-type ESBL, particularly CTX-M-15, supporting its worldwide dissemination, including in areas with limited access to third-generation cephalosporins. This finding is a matter of concern for clinical management as third-generation cephalosporins are an alternative for treating severe cases of multidrug-resistant infections in this community.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
ESBL; Klebsiella; CTX-M-15; Bloodstream infection; Urinary tract infection; Low-income countries
ISSN:
2213-7165

Full metadata record

DC FieldValue Language
dc.contributor.authorPons, Maria J.es_PE
dc.contributor.authorVubil, Delfinoes_PE
dc.contributor.authorGuiral, Elisabetes_PE
dc.contributor.authorJaintilal, Dinises_PE
dc.contributor.authorFraile, Oscares_PE
dc.contributor.authorSoto, Sara M.es_PE
dc.contributor.authorSigauque, Betueles_PE
dc.contributor.authorNhampossa, Taciltaes_PE
dc.contributor.authorAide, Pedroes_PE
dc.contributor.authorAlonso, Pedro L.es_PE
dc.contributor.authorVila, Jordies_PE
dc.contributor.authorMandomando, Inacioes_PE
dc.contributor.authorRuiz, Joaquimes_PE
dc.date.accessioned2015-03-23T15:54:35Zes_PE
dc.date.available2015-03-23T15:54:35Zes_PE
dc.date.issued2015-03-23es_PE
dc.identifier.citationPons MJ, Vubil D, Guiral E, Jaintilal D, Fraile O, Soto SM, et al. Characterisation of extended-spectrum β-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique. J Glob Antimicrob Resist [Internet]. 2015;3:19–25. Available from: http://linkinghub.elsevier.com/retrieve/pii/S2213716515000065es_PE
dc.identifier.issn2213-7165es_PE
dc.identifier.doi10.1016/j.jgar.2015.01.004es_PE
dc.identifier.urihttp://hdl.handle.net/10757/347017es_PE
dc.description.abstractThe aim of this study was to determine the prevalence of extended-spectrum b-lactamase (ESBL)- producing Klebsiella pneumoniae isolated from urinary tract and bloodstream infections in a rural hospital in Manhic¸a, Mozambique. ESBLs were investigated among ceftriaxone-non-susceptible K. pneumoniae clinical isolates recovered between 2004 and 2009. Characterisation of blaCTX-M, blaSHV, blaOXA and blaTEM genes was performed by PCR and sequencing. Epidemiological relationships were established by phylogenetic analysis, repetitive extragenic palindromic PCR (REP-PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), whilst plasmid transferability was evaluated by conjugation. In addition,the presence of class 1 and 2 integrons was studied.A total of 19 K. pneumoniae were analysed. The blaCTX-M-15 gene was found in all strains. Other ESBL genes were found concomitantly, including blaSHV-5, blaSHV-2, blaSHV-2A, blaSHV-12 and blaSHV-38. In addition, other b-lactamases such as blaTEM-1 and blaOXA-30 were also detected. REP-PCR identified 15 different epidemiological profiles. MLST analysis also showed great variability of sequence types. The blaCTX-M-15 gene showed a high transfer capacity. The presence of class 1 integrons was high. High levels of multidrug resistance were also found. In conclusion, these data show the dominance of the CTX-M-type ESBL, particularly CTX-M-15, supporting its worldwide dissemination, including in areas with limited access to third-generation cephalosporins. This finding is a matter of concern for clinical management as third-generation cephalosporins are an alternative for treating severe cases of multidrug-resistant infections in this community.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherElsevier B.V.es_PE
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S2213716515000065es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectESBLes_PE
dc.subjectKlebsiellaes_PE
dc.subjectCTX-M-15es_PE
dc.subjectBloodstream infectiones_PE
dc.subjectUrinary tract infectiones_PE
dc.subjectLow-income countrieses_PE
dc.titleCharacterisation of extended-spectrum b-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambiquees_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalJournal of Global Antimicrobial Resistancees_PE
dc.description.peer-reviewRevisión por pareses_PE
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