Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: a systematic review

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Hdl Handle:
http://hdl.handle.net/10757/345286
Title:
Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: a systematic review
Authors:
Lloyd, Aaron; Pasupuleti, Vinay; Thota, Priyaleela; Pant, Chaitanya; Rolston, David D.K; Hernández, Adrian V. ( 0000-0002-9999-4003 ) ; Benites Zapata, Vicente A.; Fraser, Thomas G.; Donskey, Curtis J.; Deshpande, Abhishek
Citation:
Lloyd A, Pasupuleti V, Thota P, Pant C, Rolston DDK, Hernandez A V., et al. Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: a systematic review. Diagn Microbiol Infect Dis [Internet]. Elsevier B.V.; 2015;81. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0732889315000437
Publisher:
Elsevier B.V.
Journal:
Diagnostic Microbiology and Infectious Disease
Issue Date:
24-Feb-2015
URI:
http://hdl.handle.net/10757/345286
DOI:
10.1016/j.diagmicrobio.2015.02.007
Additional Links:
http://ezproxy.concytec.gob.pe:2063/science/article/pii/S0732889315000437
Abstract:
Loop-mediated isothermal DNA amplification (LAMP) are currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6,979 samples) that met inclusion criteria. When TC was used as the gold standard (6,572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95%CI, 0.93-0.97; I2 = 67.4) and a mean specificity of 0.99 (95%CI, 0.96-1.00; I2 = 97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should however be interpreted only in the presence of clinical suspicion and symptoms of CDI.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
C. difficile; LAMP; Diagnostic accuracy; Sensitivity; CDI
ISSN:
0732-8893

Full metadata record

DC FieldValue Language
dc.contributor.authorLloyd, Aarones_PE
dc.contributor.authorPasupuleti, Vinayes_PE
dc.contributor.authorThota, Priyaleelaes_PE
dc.contributor.authorPant, Chaitanyaes_PE
dc.contributor.authorRolston, David D.Kes_PE
dc.contributor.authorHernández, Adrian V.es_PE
dc.contributor.authorBenites Zapata, Vicente A.es_PE
dc.contributor.authorFraser, Thomas G.es_PE
dc.contributor.authorDonskey, Curtis J.es_PE
dc.contributor.authorDeshpande, Abhishekes_PE
dc.date.accessioned2015-02-25T04:53:40Zes_PE
dc.date.available2015-02-25T04:53:40Zes_PE
dc.date.issued2015-02-24es_PE
dc.identifier.citationLloyd A, Pasupuleti V, Thota P, Pant C, Rolston DDK, Hernandez A V., et al. Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: a systematic review. Diagn Microbiol Infect Dis [Internet]. Elsevier B.V.; 2015;81. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0732889315000437eng
dc.identifier.issn0732-8893es_PE
dc.identifier.doi10.1016/j.diagmicrobio.2015.02.007es_PE
dc.identifier.urihttp://hdl.handle.net/10757/345286es_PE
dc.description.abstractLoop-mediated isothermal DNA amplification (LAMP) are currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6,979 samples) that met inclusion criteria. When TC was used as the gold standard (6,572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95%CI, 0.93-0.97; I2 = 67.4) and a mean specificity of 0.99 (95%CI, 0.96-1.00; I2 = 97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should however be interpreted only in the presence of clinical suspicion and symptoms of CDI.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherElsevier B.V.es_PE
dc.relation.urlhttp://ezproxy.concytec.gob.pe:2063/science/article/pii/S0732889315000437es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectC. difficilees_PE
dc.subjectLAMPes_PE
dc.subjectDiagnostic accuracyes_PE
dc.subjectSensitivityes_PE
dc.subjectCDIes_PE
dc.titleAccuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: a systematic reviewes_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.journalDiagnostic Microbiology and Infectious Diseasees_PE
dc.description.peer-reviewRevisión por pareses_PE
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