Etiological and molecular diagnostic of Carrion’s disease in patients from Cajamarca - Perú

2.50
Hdl Handle:
http://hdl.handle.net/10757/323395
Title:
Etiological and molecular diagnostic of Carrion’s disease in patients from Cajamarca - Perú
Authors:
Ruiz, J; Silva, W.; Tinoco, C.; Pons, Maria J ( 0000-0001-8384-2315 ) ; Pons, Maria J ( 0000-0001-8384-2315 ) ; Del Valle, Luis J.; Gomez, C.; Bazan, Jorge; Vargas, M.; Champin, D.; Mendoza, J. del Valle
Citation:
International Journal of Infectious Diseases 16S (2012) e158–e316
Journal:
International Journal of Infectious Diseases
Issue Date:
17-Jul-2014
URI:
http://hdl.handle.net/10757/323395
DOI:
http://dx.doi.org/10.1016/j.ijid.2012.05.889
Additional Links:
http://www.sciencedirect.com/science/article/pii/S1201971212010417
Abstract:
Background: Bartonellagenus is a group of facultative intracellular pathogens that posses able to survive and proliferate inside of erythrocytes. Classified within this genus,Bartonella bacilliformisis of special relevance. This microorganism is the etiological agent of the so called Carrion’s Disease (Human bartonellosis). Additionally the presence of sub-clinical cases (asymptomatyc carriers) is of special interest, because acts as a reservoir of this illness. Carrión’s Disease is an endemic illnes in Perú, affecting in a special manner the north interandean valleys. However, the current in use diagnostic techniques (Giemsa Stain) possess low sensitivity and specificity, and due to the fact thatB. bacilliformispossess a low growth (weeks), bacterial cultures lacks of clinical utility. Thus suspictious cases frequently are not confirmed, and the real relevance of this illness remains underestimated. This work is addressed to the direct identification from blood samples ofBartonella baciliformisusing a conventional PCR. All patients were from the Cajamarca area being enrolled by the Epidemiological Surveillance program of DIRESA. Methods: The samples were processed at arriving to the laboratory, by molecular and microbiological techniques. Thus samples were cultured in Blood Columbia Agar (10%), in anaerobic conditions at 28 ◦C for a period of 2 months. Positive cultures were both Giemsa stained and identified by the amplification of a fragment the 16S rRNA gene. Genetic material was directly extracted from blood samples using the Kit High Pure (Roche diagnostic), and a fragment of 438 bp of the 16S rRNA gene was amplified withBartonellagenus specific primers. All positive PCR were sequenced (Macrogen-Korea). Results: A total of 134 blood samples were processed, from this 12 (8.9%) grown in blood agar, while in 18 (13.4%), including the aforementioned 12, the 16 s rRNA gene was amplified. In all cases the sequence analysis showed the presence ofB. bacilliformis Conclusion: Although microbiological culture is the gold standard in the identification ofBartonellaspp., this technique possess strong limitations due to the low growth of these microorganisms. However, the PCR is a rapid technique, possessing a high sensibility and specificity that may be used as routine diagnostic tool for the identification of Carrion’s Disease.
Type:
info:eu-repo/semantics/conferenceObject
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Description:
[EN] Poster presented in the poster session in the 15th ICID Abstracts. June 13th-16th 2012, Bangkok, Thailand. Session: Emerging Infectious Diseases. Date: Friday, June 15, 2012. Room: Poster & Exhibition Area.
Keywords:
Enfermedad de Carrion; Carrion’s disease; Cajamarca; Perú
Email:
juana.delvalle@upc.edu.pe

Full metadata record

DC FieldValue Language
dc.contributor.authorRuiz, Jes_PE
dc.contributor.authorSilva, W.es_PE
dc.contributor.authorTinoco, C.es_PE
dc.contributor.authorPons, Maria Jes_PE
dc.contributor.authorPons, Maria Jes_PE
dc.contributor.authorDel Valle, Luis J.es_PE
dc.contributor.authorGomez, C.es_PE
dc.contributor.authorBazan, Jorgees_PE
dc.contributor.authorVargas, M.es_PE
dc.contributor.authorChampin, D.es_PE
dc.contributor.authorMendoza, J. del Vallees_PE
dc.date.accessioned2014-07-18T00:19:23Zes_PE
dc.date.available2014-07-18T00:19:23Zes_PE
dc.date.issued2014-07-17es_PE
dc.identifier.citationInternational Journal of Infectious Diseases 16S (2012) e158–e316eng
dc.identifier.doihttp://dx.doi.org/10.1016/j.ijid.2012.05.889eng
dc.identifier.urihttp://hdl.handle.net/10757/323395es_PE
dc.description[EN] Poster presented in the poster session in the 15th ICID Abstracts. June 13th-16th 2012, Bangkok, Thailand. Session: Emerging Infectious Diseases. Date: Friday, June 15, 2012. Room: Poster & Exhibition Area.eng
dc.description.abstractBackground: Bartonellagenus is a group of facultative intracellular pathogens that posses able to survive and proliferate inside of erythrocytes. Classified within this genus,Bartonella bacilliformisis of special relevance. This microorganism is the etiological agent of the so called Carrion’s Disease (Human bartonellosis). Additionally the presence of sub-clinical cases (asymptomatyc carriers) is of special interest, because acts as a reservoir of this illness. Carrión’s Disease is an endemic illnes in Perú, affecting in a special manner the north interandean valleys. However, the current in use diagnostic techniques (Giemsa Stain) possess low sensitivity and specificity, and due to the fact thatB. bacilliformispossess a low growth (weeks), bacterial cultures lacks of clinical utility. Thus suspictious cases frequently are not confirmed, and the real relevance of this illness remains underestimated. This work is addressed to the direct identification from blood samples ofBartonella baciliformisusing a conventional PCR. All patients were from the Cajamarca area being enrolled by the Epidemiological Surveillance program of DIRESA. Methods: The samples were processed at arriving to the laboratory, by molecular and microbiological techniques. Thus samples were cultured in Blood Columbia Agar (10%), in anaerobic conditions at 28 ◦C for a period of 2 months. Positive cultures were both Giemsa stained and identified by the amplification of a fragment the 16S rRNA gene. Genetic material was directly extracted from blood samples using the Kit High Pure (Roche diagnostic), and a fragment of 438 bp of the 16S rRNA gene was amplified withBartonellagenus specific primers. All positive PCR were sequenced (Macrogen-Korea). Results: A total of 134 blood samples were processed, from this 12 (8.9%) grown in blood agar, while in 18 (13.4%), including the aforementioned 12, the 16 s rRNA gene was amplified. In all cases the sequence analysis showed the presence ofB. bacilliformis Conclusion: Although microbiological culture is the gold standard in the identification ofBartonellaspp., this technique possess strong limitations due to the low growth of these microorganisms. However, the PCR is a rapid technique, possessing a high sensibility and specificity that may be used as routine diagnostic tool for the identification of Carrion’s Disease.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1201971212010417es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectEnfermedad de Carriones_PE
dc.subjectCarrion’s diseasees_PE
dc.subjectCajamarcaes_PE
dc.subjectPerúes_PE
dc.titleEtiological and molecular diagnostic of Carrion’s disease in patients from Cajamarca - Perúes_PE
dc.typeinfo:eu-repo/semantics/conferenceObjectes_PE
dc.identifier.journalInternational Journal of Infectious Diseaseses_PE
dc.description.peer-reviewRevisión por pareseng
dc.contributor.emailjuana.delvalle@upc.edu.pees_PE
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