Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections

2.50
Hdl Handle:
http://hdl.handle.net/10757/322342
Title:
Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections
Authors:
Del Valle, Luis J.; Jaramillo, Michael L.; Talledo, Miguel; Pons, Maria J.; Flores, Lidia; Quispe, Ruth L.; Ramírez, Pablo; García de la Guarda, Ruth; Alvarado, Débora; Espinoza-Culupú, Abraham; Del Valle Mendoza, Juana; Vargas, Martha; Ruíz, Joaquim
Publisher:
Horizon Research Publishing
Journal:
Universal Journal of Microbiology Research
Issue Date:
2-Jul-2014
URI:
http://hdl.handle.net/10757/322342
DOI:
10.13189/ujmr.2014.020102
Additional Links:
http://www.hrpub.org/download/20131215/UJMR2-10201796.pdf
Abstract:
Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Bartonella; PCR-RFLP; 16s rRNA Gene; Identification
ISSN:
2331-6721
EISSN:
2331-673X

Full metadata record

DC FieldValue Language
dc.contributor.authorDel Valle, Luis J.es_PE
dc.contributor.authorJaramillo, Michael L.es_PE
dc.contributor.authorTalledo, Migueles_PE
dc.contributor.authorPons, Maria J.es_PE
dc.contributor.authorFlores, Lidiaes_PE
dc.contributor.authorQuispe, Ruth L.es_PE
dc.contributor.authorRamírez, Pabloes_PE
dc.contributor.authorGarcía de la Guarda, Ruthes_PE
dc.contributor.authorAlvarado, Déboraes_PE
dc.contributor.authorEspinoza-Culupú, Abrahames_PE
dc.contributor.authorDel Valle Mendoza, Juanaes_PE
dc.contributor.authorVargas, Marthaes_PE
dc.contributor.authorRuíz, Joaquimes_PE
dc.date.accessioned2014-07-03T03:09:59Z-
dc.date.available2014-07-03T03:09:59Z-
dc.date.issued2014-07-02-
dc.identifier.issn2331-6721-
dc.identifier.doi10.13189/ujmr.2014.020102-
dc.identifier.urihttp://hdl.handle.net/10757/322342es_PE
dc.description.abstractAbstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherHorizon Research Publishinges_PE
dc.relation.urlhttp://www.hrpub.org/download/20131215/UJMR2-10201796.pdfes_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectBartonellaes_PE
dc.subjectPCR-RFLPes_PE
dc.subject16s rRNA Genees_PE
dc.subjectIdentificationes_PE
dc.titleDevelopment of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infectionses_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.eissn2331-673X-
dc.identifier.journalUniversal Journal of Microbiology Researches_PE
All Items in UPC are protected by copyright, with all rights reserved, unless otherwise indicated.