Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections

2.50
Hdl Handle:
http://hdl.handle.net/10757/322342
Title:
Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections
Authors:
Del Valle, Luis J.; Jaramillo, Michael L.; Talledo, Miguel; Pons, Maria J.; Flores, Lidia; Quispe, Ruth L.; Ramírez, Pablo; García de la Guarda, Ruth; Alvarado, Débora; Espinoza-Culupú, Abraham; Del Valle Mendoza, Juana; Vargas, Martha; Ruíz, Joaquim
Publisher:
Horizon Research Publishing
Journal:
Universal Journal of Microbiology Research
Issue Date:
2-Jul-2014
URI:
http://hdl.handle.net/10757/322342
DOI:
10.13189/ujmr.2014.020102
Additional Links:
http://www.hrpub.org/download/20131215/UJMR2-10201796.pdf
Abstract:
Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Keywords:
Bartonella; PCR-RFLP; 16s rRNA Gene; Identification
ISSN:
2331-6721
EISSN:
2331-673X

Full metadata record

DC FieldValue Language
dc.contributor.authorDel Valle, Luis J.spa
dc.contributor.authorJaramillo, Michael L.spa
dc.contributor.authorTalledo, Miguelspa
dc.contributor.authorPons, Maria J.spa
dc.contributor.authorFlores, Lidiaspa
dc.contributor.authorQuispe, Ruth L.spa
dc.contributor.authorRamírez, Pablospa
dc.contributor.authorGarcía de la Guarda, Ruthspa
dc.contributor.authorAlvarado, Déboraspa
dc.contributor.authorEspinoza-Culupú, Abrahamspa
dc.contributor.authorDel Valle Mendoza, Juanaspa
dc.contributor.authorVargas, Marthaspa
dc.contributor.authorRuíz, Joaquimspa
dc.date.accessioned2014-07-03T03:09:59Z-
dc.date.available2014-07-03T03:09:59Z-
dc.date.issued2014-07-02-
dc.identifier.issn2331-6721-
dc.identifier.doi10.13189/ujmr.2014.020102-
dc.identifier.urihttp://hdl.handle.net/10757/322342-
dc.description.abstractAbstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.eng
dc.formatapplication/pdfspa
dc.language.isoengeng
dc.publisherHorizon Research Publishingspa
dc.relation.urlhttp://www.hrpub.org/download/20131215/UJMR2-10201796.pdfspa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.subjectBartonellaspa
dc.subjectPCR-RFLPspa
dc.subject16s rRNA Genespa
dc.subjectIdentificationspa
dc.titleDevelopment of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infectionsspa
dc.typeinfo:eu-repo/semantics/articlespa
dc.identifier.eissn2331-673X-
dc.identifier.journalUniversal Journal of Microbiology Researchspa
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