Phylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian children

2.50
Hdl Handle:
http://hdl.handle.net/10757/320507
Title:
Phylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian children
Authors:
Contreras, C. A.; Ochoa, T. J.; Ruiz, J.; Lacher, D. W.; Rivera, F. P.; Saenz, Y.; Chea-Woo, E.; Zavaleta, N.; Gil, A. I.; Lanata, C. F.
Publisher:
Society for General Microbiology (SGM)
Journal:
Journal of Medical Microbiology
Issue Date:
10-Jun-2014
URI:
http://hdl.handle.net/10757/320507
DOI:
10.1099/jmm.0.026666-0
Additional Links:
http://jmm.sgmjournals.org/content/60/5/639
Abstract:
The aim of this study was to determine the prevalence, virulence factors (stx, eae, ehxA and astA) and phylogenetic relationships [PFGE and multilocus sequence typing (MLST)] of Shiga toxinproducing Escherichia coli (STEC) strains isolated from four previous cohort studies in 2212 Peruvian children aged ,36 months. STEC prevalence was 0.4% (14/3219) in diarrhoeal and 0.6% (15/2695) in control samples. None of the infected children developed haemolytic uraemic syndrome (HUS) or other complications of STEC. stx1 was present in 83% of strains, stx2 in 17 %, eae in 72 %, ehxA in 59% and astA in 14 %. The most common serotype was O26: H11 (14%) and the most common seropathotype was B (45 %). The strains belonged mainly to phylogenetic group B1 (52 %). The distinct combinations of alleles across the seven MLST loci were used to define 13 sequence types among 19 STEC strains. PFGE typing of 20 STEC strains resulted in 19 pulsed-field patterns. Comparison of the patterns revealed 11 clusters (I–XI), each usually including strains belonging to different serotypes; one exception was cluster VI, which gathered exclusively seven strains of seropathotype B, clonal group enterohaemorrhagic E. coli (EHEC) 2 and phylogenetic group B1. In summary, STEC prevalence was low in Peruvian children with diarrhoea in the community setting. The strains were phylogenetically diverse and associated with mild infections. However, additional studies are needed in children with bloody diarrhoea and HUS.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
ISSN:
0022-2615
EISSN:
1473-5644
Sponsors:
Agencia Espan˜ola de Cooperacio´n Internacional (AECID), Spain, Programa de Cooperacio´n Interuniversitaria e Investigacio´n Cientı´fica con Iberoame´rica (D/019499/08 and D/024648/09); Institutional Research Funds from Universidad Peruana Cayetano Heredia, Peru´ (T. J. O.) and Instituto Nacional de Salud del Nin˜o, Peru (L. H.); Military Infectious Disease Research Program work unit # 60000.000.0.B0017 (R. C. M.); Instituto de Investigacio´n Nutricional, Peru (N. Z. and C. F. L.); Programa Miguel Servet (CP05/00130) (J. R.); and the National Institutes of Health, USA, Public Health Service awards 1K01TW007405 (T. J. O.) and R01-HD051716 (T. G. C. and E. C.-W.).

Full metadata record

DC FieldValue Language
dc.contributor.authorContreras, C. A.es_PE
dc.contributor.authorOchoa, T. J.es_PE
dc.contributor.authorRuiz, J.es_PE
dc.contributor.authorLacher, D. W.es_PE
dc.contributor.authorRivera, F. P.es_PE
dc.contributor.authorSaenz, Y.es_PE
dc.contributor.authorChea-Woo, E.es_PE
dc.contributor.authorZavaleta, N.es_PE
dc.contributor.authorGil, A. I.es_PE
dc.contributor.authorLanata, C. F.es_PE
dc.date.accessioned2014-06-11T04:09:32Z-
dc.date.available2014-06-11T04:09:32Z-
dc.date.issued2014-06-10-
dc.identifier.issn0022-2615-
dc.identifier.doi10.1099/jmm.0.026666-0-
dc.identifier.urihttp://hdl.handle.net/10757/320507es_PE
dc.description.abstractThe aim of this study was to determine the prevalence, virulence factors (stx, eae, ehxA and astA) and phylogenetic relationships [PFGE and multilocus sequence typing (MLST)] of Shiga toxinproducing Escherichia coli (STEC) strains isolated from four previous cohort studies in 2212 Peruvian children aged ,36 months. STEC prevalence was 0.4% (14/3219) in diarrhoeal and 0.6% (15/2695) in control samples. None of the infected children developed haemolytic uraemic syndrome (HUS) or other complications of STEC. stx1 was present in 83% of strains, stx2 in 17 %, eae in 72 %, ehxA in 59% and astA in 14 %. The most common serotype was O26: H11 (14%) and the most common seropathotype was B (45 %). The strains belonged mainly to phylogenetic group B1 (52 %). The distinct combinations of alleles across the seven MLST loci were used to define 13 sequence types among 19 STEC strains. PFGE typing of 20 STEC strains resulted in 19 pulsed-field patterns. Comparison of the patterns revealed 11 clusters (I–XI), each usually including strains belonging to different serotypes; one exception was cluster VI, which gathered exclusively seven strains of seropathotype B, clonal group enterohaemorrhagic E. coli (EHEC) 2 and phylogenetic group B1. In summary, STEC prevalence was low in Peruvian children with diarrhoea in the community setting. The strains were phylogenetically diverse and associated with mild infections. However, additional studies are needed in children with bloody diarrhoea and HUS.eng
dc.description.sponsorshipAgencia Espan˜ola de Cooperacio´n Internacional (AECID), Spain, Programa de Cooperacio´n Interuniversitaria e Investigacio´n Cientı´fica con Iberoame´rica (D/019499/08 and D/024648/09); Institutional Research Funds from Universidad Peruana Cayetano Heredia, Peru´ (T. J. O.) and Instituto Nacional de Salud del Nin˜o, Peru (L. H.); Military Infectious Disease Research Program work unit # 60000.000.0.B0017 (R. C. M.); Instituto de Investigacio´n Nutricional, Peru (N. Z. and C. F. L.); Programa Miguel Servet (CP05/00130) (J. R.); and the National Institutes of Health, USA, Public Health Service awards 1K01TW007405 (T. J. O.) and R01-HD051716 (T. G. C. and E. C.-W.).eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherSociety for General Microbiology (SGM)es_PE
dc.relation.urlhttp://jmm.sgmjournals.org/content/60/5/639es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.titlePhylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian childrenes_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.eissn1473-5644-
dc.identifier.journalJournal of Medical Microbiologyes_PE
dc.description.peer-reviewRevisión por pareses_PE
All Items in UPC are protected by copyright, with all rights reserved, unless otherwise indicated.