Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children.

2.50
Hdl Handle:
http://hdl.handle.net/10757/314308
Title:
Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children.
Authors:
Contreras, C. A.; Ochoa, T. J.; Ruiz, J.; Lacher, D. W.; Durand, D.; DebRoy, C.; Lanata, C. F.; Cleary, T. G.
Citation:
J Med Microbiol. 2012 Aug;61(Pt 8):1114-20
Publisher:
Society for General Microbiology
Issue Date:
19-Mar-2014
URI:
http://hdl.handle.net/10757/314308
DOI:
10.1099/jmm.0.045443-0
Additional Links:
http://jmm.sgmjournals.org/content/61/Pt_8/1114.full; http://www.ncbi.nlm.nih.gov/pubmed/22493278
Abstract:
The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (P,0.001); 28.9% of diarrhoeal and 17.6% of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P,0.05). espD-alpha was more common among acute episodes (P,0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
ISSN:
0022-2615
EISSN:
1473-5644
Sponsors:
This work was partially funded by the National Institutes of Health, USA, Public Health Service awards 1K01TW007405 (T.J.O) and R01- HD051716 (T.G.C); Agencia Espan˜ola de Cooperacio´n Internacional (AECID), Spain, Programa de Cooperacio´n Interuniversitaria e Investigacio´n Cientı´fica con Iberoame´rica (D/019499/08, D/024648/ 09 and D/030509/10); and Fondo de Investigaciones Sanitarias, Spain [J.R. has a Miguel Servet fellowship (CP05/0130)].

Full metadata record

DC FieldValue Language
dc.contributor.authorContreras, C. A.spa
dc.contributor.authorOchoa, T. J.spa
dc.contributor.authorRuiz, J.spa
dc.contributor.authorLacher, D. W.spa
dc.contributor.authorDurand, D.spa
dc.contributor.authorDebRoy, C.spa
dc.contributor.authorLanata, C. F.spa
dc.contributor.authorCleary, T. G.spa
dc.date.accessioned2014-03-20T02:19:38Z-
dc.date.available2014-03-20T02:19:38Z-
dc.date.issued2014-03-19-
dc.identifier.citationJ Med Microbiol. 2012 Aug;61(Pt 8):1114-20spa
dc.identifier.issn0022-2615-
dc.identifier.doi10.1099/jmm.0.045443-0eng
dc.identifier.urihttp://hdl.handle.net/10757/314308-
dc.description.abstractThe aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (P,0.001); 28.9% of diarrhoeal and 17.6% of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P,0.05). espD-alpha was more common among acute episodes (P,0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.eng
dc.description.sponsorshipThis work was partially funded by the National Institutes of Health, USA, Public Health Service awards 1K01TW007405 (T.J.O) and R01- HD051716 (T.G.C); Agencia Espan˜ola de Cooperacio´n Internacional (AECID), Spain, Programa de Cooperacio´n Interuniversitaria e Investigacio´n Cientı´fica con Iberoame´rica (D/019499/08, D/024648/ 09 and D/030509/10); and Fondo de Investigaciones Sanitarias, Spain [J.R. has a Miguel Servet fellowship (CP05/0130)].eng
dc.formatapplication/pdfspa
dc.language.isoengeng
dc.publisherSociety for General Microbiologyspa
dc.relation.urlhttp://jmm.sgmjournals.org/content/61/Pt_8/1114.fullspa
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22493278spa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.titleGenetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children.spa
dc.typeinfo:eu-repo/semantics/articlespa
dc.identifier.eissn1473-5644-
dc.description.peer-reviewRevisión por paresspa
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