Fecal Leukocytes in Children Infected with Diarrheagenic Escherichia coli

2.50
Hdl Handle:
http://hdl.handle.net/10757/314005
Title:
Fecal Leukocytes in Children Infected with Diarrheagenic Escherichia coli
Authors:
Mercado, Erik H.; Ochoa, Theresa J.; Ecker, Lucie; Cabello, Martin; Durand, David; Barletta, Francesca; Molina, Margarita; Gil, Ana I.; Huicho, Luis; Lanata, Claudio F.; Cleary, Thomas G.
Citation:
JOURNAL OF CLINICAL MICROBIOLOGY, Apr. 2011, p. 1376–1381
Publisher:
American Society for Microbiology
Issue Date:
12-Mar-2014
URI:
http://hdl.handle.net/10757/314005
DOI:
doi:10.1128/JCM.02199-10
Additional Links:
http://jcm.asm.org/content/early/2011/02/16/JCM.02199-10
Abstract:
The purpose of this study was to determine the presence and quantity of fecal leukocytes in children infected with diarrheagenic Escherichia coli and to compare these levels between diarrhea and control cases. We analyzed 1,474 stool samples from 935 diarrhea episodes and 539 from healthy controls of a cohort study of children younger than 2 years of age in Lima, Peru. Stools were analyzed for common enteric pathogens, and diarrheagenic E. coli isolates were studied by a multiplex real-time PCR. Stool smears were stained with methylene blue and read by a blinded observer to determine the number of polymorphonuclear leukocytes per high-power field (L/hpf). Fecal leukocytes at >10 L/hpf were present in 11.8% (110/935) of all diarrheal episodes versus 1.1% (6/539) in controls (P < 0.001). Among stool samples with diarrheagenic E. coli as the only pathogen isolated (excluding coinfection), fecal leukocytes at >10 L/hpf were present in 8.5% (18/212) of diarrhea versus 1.3% (2/157) of control samples (P < 0.01). Ninety-five percent of 99 diarrheagenic E. coli diarrhea samples were positive for fecal lactoferrin. Adjusting for the presence of blood in stools, age, sex, undernutrition, and breastfeeding, enterotoxigenic E. coli (ETEC) isolation as a single pathogen, excluding coinfections, was highly associated with the presence of fecal leukocytes (>10 L/hpf) with an odds ratio (OR) of 4.1 (95% confidence interval [CI], 1.08 to 15.51; P < 0.05). Although diarrheagenic E. coli was isolated with similar frequencies in diarrhea and control samples, clearly it was associated with a more inflammatory response during symptomatic infection; however, in general, these pathogens elicited a mild inflammatory response.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
ISSN:
0095-1137
EISSN:
1098-660X; 0095-1137

Full metadata record

DC FieldValue Language
dc.contributor.authorMercado, Erik H.es_PE
dc.contributor.authorOchoa, Theresa J.es_PE
dc.contributor.authorEcker, Luciees_PE
dc.contributor.authorCabello, Martines_PE
dc.contributor.authorDurand, Davides_PE
dc.contributor.authorBarletta, Francescaes_PE
dc.contributor.authorMolina, Margaritaes_PE
dc.contributor.authorGil, Ana I.es_PE
dc.contributor.authorHuicho, Luises_PE
dc.contributor.authorLanata, Claudio F.es_PE
dc.contributor.authorCleary, Thomas G.es_PE
dc.date.accessioned2014-03-13T02:55:29Z-
dc.date.available2014-03-13T02:55:29Z-
dc.date.issued2014-03-12-
dc.identifier.citationJOURNAL OF CLINICAL MICROBIOLOGY, Apr. 2011, p. 1376–1381es_PE
dc.identifier.issn0095-1137-
dc.identifier.doidoi:10.1128/JCM.02199-10-
dc.identifier.urihttp://hdl.handle.net/10757/314005es_PE
dc.description.abstractThe purpose of this study was to determine the presence and quantity of fecal leukocytes in children infected with diarrheagenic Escherichia coli and to compare these levels between diarrhea and control cases. We analyzed 1,474 stool samples from 935 diarrhea episodes and 539 from healthy controls of a cohort study of children younger than 2 years of age in Lima, Peru. Stools were analyzed for common enteric pathogens, and diarrheagenic E. coli isolates were studied by a multiplex real-time PCR. Stool smears were stained with methylene blue and read by a blinded observer to determine the number of polymorphonuclear leukocytes per high-power field (L/hpf). Fecal leukocytes at >10 L/hpf were present in 11.8% (110/935) of all diarrheal episodes versus 1.1% (6/539) in controls (P < 0.001). Among stool samples with diarrheagenic E. coli as the only pathogen isolated (excluding coinfection), fecal leukocytes at >10 L/hpf were present in 8.5% (18/212) of diarrhea versus 1.3% (2/157) of control samples (P < 0.01). Ninety-five percent of 99 diarrheagenic E. coli diarrhea samples were positive for fecal lactoferrin. Adjusting for the presence of blood in stools, age, sex, undernutrition, and breastfeeding, enterotoxigenic E. coli (ETEC) isolation as a single pathogen, excluding coinfections, was highly associated with the presence of fecal leukocytes (>10 L/hpf) with an odds ratio (OR) of 4.1 (95% confidence interval [CI], 1.08 to 15.51; P < 0.05). Although diarrheagenic E. coli was isolated with similar frequencies in diarrhea and control samples, clearly it was associated with a more inflammatory response during symptomatic infection; however, in general, these pathogens elicited a mild inflammatory response.eng
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherAmerican Society for Microbiologyes_PE
dc.relation.urlhttp://jcm.asm.org/content/early/2011/02/16/JCM.02199-10es_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.titleFecal Leukocytes in Children Infected with Diarrheagenic Escherichia colies_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.eissn1098-660X-
dc.identifier.eissn0095-1137eng
dc.description.peer-reviewRevisión por pareses_PE
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