Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes

2.50
Hdl Handle:
http://hdl.handle.net/10757/313901
Title:
Diarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypes
Authors:
Weilg Espejo, Pablo; Orellana Peralta, Fiorella; Cornejo Pacheres, Hernán; Del Valle, Luis J.; Cornejo Tapia, Ángela; Bazán Mayra, Jorge; Ruiz, Joaquim; Del Valle Mendoza, Juana
Citation:
Trans R Soc Trop Med Hyg first published online April 27, 2014. doi:10.1093/trstmh/tru059
Publisher:
Oxford University Press
Issue Date:
10-Mar-2014
URI:
http://hdl.handle.net/10757/313901
DOI:
10.1093/trstmh/tru059
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/24778205
Abstract:
Background: Gastroenteritis by rotavirus is responsible for approximately 810 annual deaths/year in children under 5 years in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular-epidemiology of rotavirus diarrhea in Peru Methodology: A total of 131 stool samples were obtained from children under 5 years old hospitalized from January 2010 to December 2012 in the Hospital Regional de Cajamarca, Peru. ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were obtained by semi-nested multiplex RT-PCR and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 18.80% (22/117) tested positive for rotavirus by ELISA and 35.90% (42/117) by RT-PCR. Among the G-genotype identified, G9 in 35.71% (15/42) and G12 in 33.33% (14/42) were the most prevalent. With the most common combination being G12/P6 in 23.81% (10/42). Conclusions: A high prevalence of the G12/P6 genotype was detected. It is know that this genotype is not covered by the current vaccines available. More in depth studies are needed to know the current rotavirus genotypes presents in Peru.
Type:
info:eu-repo/semantics/article
Rights:
info:eu-repo/semantics/openAccess
Language:
eng
Description:
Artículo sustentado el 30 de Enero 2014 para la obtención del título profesional Médico Cirujano en la Escuela de Medicina, Facultad de Ciencias de la Salud. Universidad Peruana de Ciencias Aplicadas - UPC.; Artículo publicado el 27 de Abril de 2014 en la Revista Transactions of the Royal Society of Tropical Medicine & Hygiene (Oxford University Press).
Keywords:
Viral genotypes; Epidemiology; Acute gastroenteritis; Peru
ISSN:
1878-3503
EISSN:
0035-9203

Full metadata record

DC FieldValue Language
dc.contributor.authorWeilg Espejo, Pabloes_PE
dc.contributor.authorOrellana Peralta, Fiorellaes_PE
dc.contributor.authorCornejo Pacheres, Hernánes_PE
dc.contributor.authorDel Valle, Luis J.es_PE
dc.contributor.authorCornejo Tapia, Ángelaes_PE
dc.contributor.authorBazán Mayra, Jorgees_PE
dc.contributor.authorRuiz, Joaquimes_PE
dc.contributor.authorDel Valle Mendoza, Juanaes_PE
dc.date.accessioned2014-03-11T03:08:32Zes_PE
dc.date.available2014-03-11T03:08:32Zes_PE
dc.date.issued2014-03-10es_PE
dc.identifier.citationTrans R Soc Trop Med Hyg first published online April 27, 2014. doi:10.1093/trstmh/tru059eng
dc.identifier.issn1878-3503es_PE
dc.identifier.doi10.1093/trstmh/tru059eng
dc.identifier.urihttp://hdl.handle.net/10757/313901es_PE
dc.descriptionArtículo sustentado el 30 de Enero 2014 para la obtención del título profesional Médico Cirujano en la Escuela de Medicina, Facultad de Ciencias de la Salud. Universidad Peruana de Ciencias Aplicadas - UPC.es_PE
dc.descriptionArtículo publicado el 27 de Abril de 2014 en la Revista Transactions of the Royal Society of Tropical Medicine & Hygiene (Oxford University Press).es_PE
dc.description.abstractBackground: Gastroenteritis by rotavirus is responsible for approximately 810 annual deaths/year in children under 5 years in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular-epidemiology of rotavirus diarrhea in Peru Methodology: A total of 131 stool samples were obtained from children under 5 years old hospitalized from January 2010 to December 2012 in the Hospital Regional de Cajamarca, Peru. ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were obtained by semi-nested multiplex RT-PCR and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 18.80% (22/117) tested positive for rotavirus by ELISA and 35.90% (42/117) by RT-PCR. Among the G-genotype identified, G9 in 35.71% (15/42) and G12 in 33.33% (14/42) were the most prevalent. With the most common combination being G12/P6 in 23.81% (10/42). Conclusions: A high prevalence of the G12/P6 genotype was detected. It is know that this genotype is not covered by the current vaccines available. More in depth studies are needed to know the current rotavirus genotypes presents in Peru.eng
dc.formatapplication/pdfeng
dc.formatapplication/mswordeng
dc.language.isoenges_PE
dc.publisherOxford University Presseng
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24778205eng
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.sourceUniversidad Peruana de Ciencias Aplicadas (UPC)es_PE
dc.sourceRepositorio Académico - UPCes_PE
dc.subjectViral genotypeseng
dc.subjectEpidemiologyeng
dc.subjectAcute gastroenteritiseng
dc.subjectPerueng
dc.titleDiarrhoea caused by rotavirus in a regional Peruvian hospital: determination of circulating genotypeseng
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.eissn0035-9203es_PE
dc.description.peer-reviewRevisión por pareses_PE
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